| 货号 | MCA51FT |
| 克隆号 | OX-18 |
| 同种亚型 | IgG1 |
| 反应种属 | Rat |
| 来源宿主 | Mouse |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC (MCA51FT) | |
Published customer image: Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 used for the detection of RT1A expressing cells in mouse brain by immunofluorescence on formalin fixed tissue cryosections. Image caption: MHCI molecules are expressed in the nucleus accumbens core in WT mice. Staining for MHCI with OX-18 antibody (upper-left), Neurogranin (upper-center) and the merged image (upper-right) are shown. Lower images represent negative controls without OX-18. Scale bar represents 50 μm. From: Edamura M, Murakami G, Meng H, Itakura M, Shigemoto R, et al. (2014) Functional Deficiency of MHC Class I Enhances LTP and Abolishes LTD in the Nucleus Accumbens of Mice. PLoS ONE 9(9): e107099. | |
Published customer image: FITC conjugated Mouse anti Rat MHC class I RT1A antibody used to evaluate MHC class I expression on rat mesenchymal stem cells by flow cytometry. Image caption: Immunophenotyping and pro-inflammatory cytokine expression of untransduced and Ad-transduced MSCs. (A) Untransduced and Ad-transduced MSCs were stained for expression of the immunologically relevant markers MHC class I/II, CD80 and CD86 with specific antibodies and analysed by flow cytometry. (B) MSCs were transduced with Ad.GFP and after 24 h cells were collected and subjected to mRNA isolation and cDNA synthesis. RT-PCR analysis showing mRNA expression levels of IL-1β, IFN-γ and IL-6 from untransduced and Ad-transduced MSCs. Data shown are means ±SD of three independent experiments. 2E-ΔCT = 2-ΔCT? number of copies of gene of interest relative to the number of copes of the internal control gene, β-actin. From: Treacy O, Ryan AE, Heinzl T, O'Flynn L, Cregg M, et al. (2012) Adenoviral Transduction of Mesenchymal Stem Cells: In Vitro Responses and In Vivo Immune Responses after Cell Transplantation. PLoS ONE 7(8): e42662. | |
Published customer image: Mouse anti Rat MHC class I RT1A antibody, clone OX-18 used for the detection of MHC classI expressing cells in rat brain by immunohistochemistry of formalin fixed tissue cryosections. Image caption: Expression of major histocompatibility complex (MHC) class I and class II antigens in the right injected striatum. Photomicrographs were prepared from coronal sections through the striatum and processed for MHC class I (A–D) and MHC class II (F–I) immunocytochemistry in representative rats of the M PBS-Str hEPO (A and F), M hEPO-Str hEPO (B and G), Str PBS-Str hEPO (C and H) and Str hEPO-Str hEPO (D and I) groups 3 weeks after the second injections. The scale bar = 100 μm in I also for A–D and F–H. High magnification photomicrographs in the left bottom were prepared from the box areas in (A) and (H). (E) and (J) Arbitrary rating scores of MHC class I (E) and MHC class II (J) immunostaining in the right injected striatum of rats from the M PBS-Str hEPO, M hEPO-Str hEPO, Str PBS-Str hEPO and Str hEPO-Str hEPO groups. The bars represent the median values of rating scores, and the circles depict the individual values. Filled circles indicate the rats from which the photomicrographs were taken. A Kruskal-Wallis test followed by Mann-Whitney U tests was used to compare the rating scores between groups. * p<0.05 versus indicated groups. From: Yang C, Yang W-H, Chen S-S, Ma B-F, Li B, et al. (2013) Pre-immunization with an Intramuscular Injection of AAV9-Human Erythropoietin Vectors Reduces the Vector-Mediated Transduction following Re-Administration in Rat Brain. PLoS ONE 8(5): e63876. |
