| 货号 | 9812S |
| 描述 | The KinomeView™ Profiling Kit provides a set of Phospho-Motif Antibodies that cover a large portion of the kinome and react broadly with serine, threonine, and tyrosine phosphorylation mediated by diverse kinase families throughout the kinome. This kit will provide researchers with an immunoblotting strategy to dissect the complexity of the phosphoproteome and determine the kinase families involved in the regulation of diverse physiological processes. By using the provided Phospho-motif Antibodies, the investigator can assess global changes in protein phosphorylation by western analysis across a range of experimental samples and conditions. |
| 目标/特异性 | Each antibody recognizes endogenous levels of the respective target motif. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 供应商 | CST |
| 背景 | Cell Signaling Technology (CST) has developed Phospho-motif Antibodies that have broad immunoreactivity for serine, threonine and tyrosine phosphorylation mediated by both known and unknown kinases. Using these Motif Antibodies for western blotting provides a Kinome-wide view of cellular phosphorylation. |
| 存放说明 | -20C |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated threonine-X-arginine motifs, using Phospho-Threonine-X-Arginine Antibody. | |
Phospho-Threonine-X-Arginine Antibody ELISA: Signal-to-noise ratio of phospho- versus nonphospho-peptides containing the phospho-threonine-X-arginine motif. (T* denotes phosphorylated threonine.) | |
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated (0.1 µM for 30 minutes prior to lysis), using Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb. | |
Immunoprecipitation of extracts from NIH/3T3 cells, untreated or treated with 100 ng/ml PDGF for 20 minutes prior to lysis, using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb and Akt Antibody #9272. Western blots were performed using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb (upper) or Akt Antibody #9272 (lower). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from Jurkat cells, untreated, TPA-treated or calyculin A-treated, using Phospho-Threonine-X-Arginine Antibody. | |
Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) | |
Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. | |
Immunoprecipitation of extracts from serum-starved NIH/3T3 cells, untreated or treated with FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb, followed by Western blot analysis using the same antibody. | |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) | |
Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded renal cell carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. | |
Western blot analysis of extracts from serum-starved A431 cells, phosphorylated in vitro with PKA kinase or treated in culture with forskolin/IBMX, using Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb. Lysis buffer: 1.0% Triton X-100 (lanes 1 and 2), 2.0% SDS (lanes 3 and 4). | |
Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) |
