| 货号 | 8356T |
| 描述 | The Death Receptor Antibody Sampler Kit provides an economical means to investigate the machinery of death receptor-mediated apoptosis. The kit includes enough of each primary antibody to perform four western mini-blot experiments per primary. |
| 目标/特异性 | Each antibody in the Death Receptor Antibody Sampler Kit detects endogenous levels of the respective target protein. The TNF-R1, DR3, DR5, and RIP antibodies do not cross-react with other related family members. |
| 供应商 | CST |
| 背景 | The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC) resulting in activation of caspases. Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (6). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (7,8). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (9). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (10,11). |
| 存放说明 | -20C |
| 参考文献 | 1 . Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. 2 . Thorburn, A. (2004) Cell Signal 16, 139-44. 3 . Hsu, H. et al. (1996) Immunity 4, 387-96. 4 . Sheridan, J.P. et al. (1997) Science 277, 818-21. 5 . Stanger, B.Z. et al. (1995) Cell 81, 513-23. 6 . Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6. 7 . Ting, A.T. et al. (1996) EMBO J 15, 6189-96. 8 . Nagata, S. (1997) Cell 88, 355-65. 9 . Pitti, R.M. et al. (1998) Nature 396, 699-703. 10 . Kelliher, M.A. et al. (1998) Immunity 8, 297-303. 11 . Lin, Y. et al. (1999) Genes Dev 13, 2514-26. |
Western blot analysis of extracts from various cell lines using DR5 (D4E9) XP® Rabbit mAb #8074. Western blot 检测多种细胞系提取物，使用抗体为DR5 (D4E9) XP® Rabbit mAb #8074。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human DcR2 (hDcR2; +), using DcR2 (D13H4) Rabbit mAb. | |
Western blot anlaysis of extracts from various cell lines using DcR2 (D13H4) Rabbit mAb. | |
Confocal immunofluorescent analysis of HT-1080 cells using DR5 (D4E9) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using DR5 (D4E9) XP® Rabbit mAb. | |
Western blot analysis of extracts from A549 cells, untreated or doxorubicin-treated (500 nM) for the indicated times, using DR5 (D4E9) XP® Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with the long isoform of human DR5 (hDR5, +), using DR5 (D4E9) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using RIP (D94C12) XP® Rabbit mAb #3493. Western blot 检测多种细胞系提取物，使用抗体为RIP (D94C12) XP® Rabbit mAb #3493。 | |
Western blot analysis of extracts from various cell lines using TNF-R2 Antibody #3727. Western blot 检测多种细胞系提取物，使用抗体为TNF-R2 Antibody #3727。 | |
Western blot analysis of extracts from Jurkat and HeLa cells, treated with 4 mM hydroxyurea or 1 µg/ml nocodazole (each for 20 hours), using FADD Antibody (Human Specific) #2782. Western blot分析Jurkat和HeLa 细胞提取物，4 mM羟基脲或1 µg/ml噻氨酯哒唑处理（每个20hr），使用抗体为FADD Antibody (Human Specific) #2782。 | |
Western blot analysis of extracts from various cell lines using TRADD (7G8) Rabbit mAb #3684. Western blot 检测多种细胞系提取物，使用抗体为TRADD (7G8) Rabbit mAb #3684。 | |
Western blot analysis of extracts from various cell lines using TNF-R1 (C25C1) Rabbit mAb #3736. Western blot 检测多种细胞系提取物，使用抗体为TNF-R1 (C25C1) Rabbit mAb #3736。 | |
Western blot analysis of extracts from various cell lines using DcR3 Antibody #4758. Western blot 检测多种细胞系提取物，使用抗体为DcR3 Antibody #4758。 | |
Western blot analysis of extracts from various cell lines using DR3 Antibody #3254. Western blot 检测多种细胞系提取物，使用抗体为DR3 Antibody #3254。 | |
Western blot analysis of extracts from COS7 cells, mock transfected or transfected with human Fas, and from ACHN and HT-1080 cell lines, using Fas (C18C12) Rabbit mAb #4233. Western blot方法检测COS7细胞提取物：空质粒转染或人Fas转染；以及 ACHN 和 HT-1080细胞系提取物，使用抗体为Fas (C18C12) Rabbit mAb #4233。 |
