| 货号 | 9328T |
| 目标/特异性 | Unless otherwise indicated, each antibody in the c-Oncogene Antibody Sampler Kit detects endogenous levels of total target protein and does not cross-react with related proteins. c-Jun (60A8) Rabbit mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state. Ras (27H5) Rabbit mAb detects endogenous levels of total K-Ras, H-Ras and N-Ras proteins. Src (36D10) Rabbit mAb detects endogenous levels of Src proteins and may cross-react with other Src family members. The c-Myc (D84C12) Rabbit mAb detects endogenous levels of total c-Myc protein. |
| 供应商 | CST |
| 背景 | The regulation of cell growth, differentiation and programmed death is coordinated by several sets of proteins that comprise essential signal transduction pathways. Many of these key regulatory proteins are encoded by proto-oncogenes, which can be activated (altered) to change the typical cell program to one of abnormal cell growth and unregulated development. Proteins encoded by proto-oncogenes include growth factors and other ligands, receptor proteins, tyrosine kinases, various regulatory proteins (i.e. GTPases) and transcription factors. Together these proteins comprise the basic elements of cell signaling pathways; altered expression or mutation of one or more of these components can lead to oncogenic growth (reviewed in 1). Non-receptor (i.e. cytoplasmic, nuclear) tyrosine kinases such as c-Abl and Src play key roles in the regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (2,3). Alteration of the corresponding c-Abl and Src proto-oncogenes is associated with oncogenesis; Abl1-BCR gene translocations result in chronic myelogenous leukemia (CML) while constitutively active Src is seen in some patients with colon cancer and altered Src expression is seen in a wide array of cancers (2,4). Regulation of Raf tyrosine kinase by Ras GTPase controls downstream kinases in the MEK/MAPK signaling pathway (5). Activation of the Ras and Raf proto-oncogenes are common in human cancers and both proteins are seen as potential therapeutic targets (6). The receptor tyrosine kinase c-Kit plays a critical role in activation and growth of hematopoietic stem cells (7); mutations that inhibit c-Kit kinase activity are associated with a variety of developmental disorders while mutations producing constitutively active c-Kit can result in mastocytosis and gastrointestinal stromal tumors (8). The alteration of key transcription factors such as c-Fos, c-Jun, c-Myc and c-Rel that are normally responsible for regulating cell and tissue growth, differentiation and the inflammation/immune response, can also result in unregulated, oncogenic cell growth (9-12).细胞生长、分化和程序性死亡的调节由一些蛋白设置协调，这些蛋白组成了重要的信号转导通路。其中许多关键的调节蛋白都是有前原癌基因编码，这些蛋白被活化后，原先典型的细胞程序改变，导致非正常的细胞生长以及发育紊乱。由原癌基因编码的蛋白包括生长因子和其他腺体、受体蛋白、酪氨酸激酶、各种调节蛋白（如GTP酶）和转录因子。这些蛋白共同组成了细胞信号转导通路的基本元件，一个或更多元件变化后的表达和突变会导致癌基因的生长（详见1中）。非受体（如胞质、胞核）酪氨酸激酶如c-Abl、Src对细胞增殖、分化、凋亡、细胞粘附和应激效应的调节起着关键作用(2,3)。相应的c-Abl、Src原癌基因的改变与癌基因的产生有关；Abl1-BCR基因的转位导致慢性粒细胞白细胞(CML)，而结构性活化Src蛋白出现于一些结肠癌患者体内，变化的Src蛋白表达出现于许多癌症疾病中(2,4)。Ras GTP酶对Ras酪氨酸激酶的调节控制了MEK/MAPK信号转导途径中的下游激酶(5)。Ras、Raf 原癌基因的活化在人类癌症中普遍存在，并且这两者都是临床治疗的潜在靶点(6)。受体酪氨酸激酶c-Kit在造血干细胞的生长和发育过程中起着重要的作用(7)；抑制c-Kit激酶活性的突变与各种发育紊乱相关，然而使c-Kit结构性活化的突变会导致肥大细胞增多症和胃肠道间质瘤(8)。c-Fos, c-Jun, c-Myc，c-Rel正常情况下会调节细胞、组织生长、分化和炎性/免疫反应，但是这些关键转录因子的改变也会导致不受控制的癌细胞生长(9-12)。 |
| 存放说明 | -20C |
| 参考文献 | 1 . Croce, C.M. (2008) N Engl J Med 358, 502-11. 2 . Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. 3 . Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83. 4 . Wang, J.Y. (2000) Oncogene 19, 5643-50. 5 . Dehm, S.M. and Bonham, K. (2004) Biochem Cell Biol 82, 263-74. 6 . Stites, E.C. et al. (2007) Science 318, 463-7. 7 . Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25. 8 . Nocka, K. et al. (1990) EMBO J 9, 1805-13. 9 . Milde-Langosch, K. (2005) Eur J Cancer 41, 2449-61. 10 . Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6. 11 . Yokota, J. et al. (1986) Science 231, 261-5. 12 . Rayet, B. and Gélinas, C. (1999) Oncogene 18, 6938-47. |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of c-Jun (60A8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using c-Myc (D84C12) XP® Rabbit mAb #5605.western blot方法检测细胞提取物：各种细胞系。使用的抗体是c-Myc (D84C12) XP® Rabbit mAb #5605。 | |
Confocal immunofluorescent analysis of NCI-H526 (left) and Jurkat (right) cells using c-Kit (D13A2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of Raji cells using c-Myc (D84C12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) XP® Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red). | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using c-Myc (D84C12) XP® Rabbit mAb. | |
Western blot analysis of extracts from H526 cells using c-Kit (D132A) XP® Rabbit mAb #3074.western blot方法检测细胞提取物：H526细胞。使用的抗体是c-Kit (D132A) XP® Rabbit mAb #3074。 | |
Western blot analysis of extracts from LNCap, TT and K-562 cells using c-Abl Antibody #2862.western blot方法检测细胞提取物：LNCap, TT，K-562细胞。使用的抗体是c-Abl Antibody #2862。 | |
Western blot analysis of extracts from HeLa, C2C12 and PC-12 cells, untreated or TPA-treated (200 nM for 30 minutes), using c-Raf Antibody #9422.western blot方法检测细胞提取物：未经处理和TPA(200 nM 30 min)处理的HeLa, C2C12，PC-12细胞。使用的抗体是c-Raf Antibody #9422。 | |
Western blot analysis of extracts from HeLa and H-4-II-E cells, serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos (9F6) Rabbit mAb #2250.western blot方法检测细胞提取物：无血清培养过夜、TPA处理4h的HeLa、H-4-II-E细胞。使用的抗体是c-Fos (9F6) Rabbit mAb #2250。 | |
Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated or UV-treated, using c-Jun (60A8) Rabbit mAb #9165.western blot方法检测细胞提取物：未经处理和紫外照射的NIH/3T3、SK-N-MC细胞。使用的抗体是c-Jun (60A8) Rabbit mAb #9165。 | |
Western blot analysis of extracts from K-562, Raji and 293 cells using c-Rel Antibody #4727.western blot方法检测细胞提取物：K-562, Raji，293细胞。使用的抗体是c-Rel Antibody #4727。 | |
Western blot analysis of extracts from 293T, C2C12 and C6 cells using Ras (27H5) Rabbit mAb #3339.western blot方法检测细胞提取物：293T, C2C12，C6 细胞。使用的抗体是Ras (27H5) Rabbit mAb #3339。 | |
Western blot analysis of extracts from various cell lines using Src (36D10) Rabbit mAb #2109.western blot方法检测细胞提取物：各种细胞系。使用的抗体是Src (36D10) Rabbit mAb #2109。 |
