| 货号 | 8218S |
| 描述 | PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CSTs product offering based upon superior performance in specified applications. |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 参考文献 | Cooper, C.S. et al. (1984) Nature 311, 29-33. Bottaro, D.P. et al. (1991) Science 251, 802-4. Bardelli, A. et al. (1997) Oncogene 15, 3103-11. Taher, T.E. et al. (2002) J Immunol 169, 3793-800. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118. |
Western blot analysis of cell extracts from HeLa cells, untreated or stimulated with HGF, using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (upper) and Met (25H2) Mouse mAb #3127 (lower). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb. | |
Immunohistochemical analysis on Src-transfected NIH/3T3 cells, using a Phospho-Src Family (Tyr416) Antibody (left) or Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (right), indicating that the antibody does not cross-react with Src phosphorylated at Tyr416 via immunohistochemistry. | |
Immunohisochemical analysis of paraffin-embedded HCC827 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb. | |
Immunohistochemical analysis of frozen MKN45 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded xenografts from 3T3-Met (left) and 3T3-Ron cells (right) using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb, indicating that this antibody does not cross-react with activated Ron by immunohistochemistry. Image courtesy of Pfizer, Inc. | |
Western blot analysis of purified active Ron kinase using a Phospho-Ron (Ser1394) Antibody (A), a Phospho-Ron (Tyr1238) Antibody (B), Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (C) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (D). This demonstrates that Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb does not cross-react with phospho-Ron by western analysis. | |
Immunohistochemical analysis of paraffin-embedded papillary renal cell carcinoma using Phospho-Met (Tyr1234/1225) (D26) XP® Rabbit mAb. | |
Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). | |
Immunohistochemical analysis of frozen MKN-45 xenograft using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
