| 货号 | 8207S |
| 描述 | PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CSTs product offering based upon superior performance in specified applications. |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 参考文献 | Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8. |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma, using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb. | |
Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (5G10) Rabbit mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal protein (Ser235/Ser236) (D57.2.2E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A549 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). | |
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb compared to a nonspecific negative control antibody (red). | |
Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). |
