| 货号 | 9942T |
| 描述 | The Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit provides an economical means to examine several members of the Bcl-2 family and their activation status. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. |
| 目标/特异性 | Each antibody in the Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other Bcl-2 family members. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112 (mouse), Ser75 (human), or Ser113 (rat). |
| 供应商 | CST |
| 背景 | The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. |
| 存放说明 | -20C |
| 参考文献 | Cory, S. et al. (2003) Oncogene 22, 8590-607. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. Yang, E. et al. (1995) Cell 80, 285-291. Zha, J. et al. (1996) Cell 87, 619-628. Datta, S. R. et al. (1997) Cell 91, 231-241. Peso, L. et al. (1997) Science 278, 687-689. Bonni, A. et al. (1999) Science 286, 1358-1362. Tan, Y. et al. (1999) J. Bio. Chem. 274, 34859-34867. Harada, H. et al. (1999) Mol. Cell 3, 413-422. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Bak (D4E4) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Bak (D4E4) Rabbit mAb. | |
Confocal immunofluorescent analysis of OVCAR8 cells using Bak (D4E4) Rabbit mAb (green; left) showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red; center). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Bax (D2E11) Rabbit mAb #5023. Brimmel et al. demonstated that Jurkat cells lack Bax protein expression (10). 使用Bax (D2E11) Rabbit mAb 兔单抗#5023对多种细胞的细胞提取物进行western blot分析。Brimmel等证明Jurkat细胞缺失Bax表达(10)。 | |
Western blot analysis of extracts from Raji, A20 and RL-7 cells using Bim (C34C5) Rabbit mAb #2933. 使用Bim (C34C5) Rabbit mAb 兔单抗#2933对Raji, A20 和RL-7细胞的细胞提取物进行western blot分析。 | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), and RS4;11 cells, untreated or okadaic acid-treated (1 µM), using BID Antibody (Human Specific). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using Bik Antibody. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Bik Antibody. | |
Western blot analysis of extracts from various cell lines using Bak (D2D3) Rabbit mAb #6947. 使用Bak (D2D3) Rabbit mAb 兔单抗#6947对多种细胞的细胞提取物，进行western blot分析。 | |
Western blot analysis of extracts from various cells using Puma Antibody #4976. | |
使用Puma Antibody 多抗#4976对多种细胞的提取物，进行western blot分析。 | |
Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb. |
