| 货号 | 12651T |
| 目标/特异性 | Unless otherwise indicated, each antibody in the PDGF Receptor Activation Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb detects endogenous levels of p44 and p42 MAP kinase when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185/Tyr187 of Erk2) and singly phosphorylated at Thr202. Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb may cross-react with activated PDGF receptor α and other protein tyrosine kinases when highly overexpressed. PDGF Receptor β (28E1) Rabbit mAb may cross-react with PDGF receptor α when highly overexpressed. Phospho-SHP-2 (Tyr542) Antibody may cross-react with activated receptor tyrosine kinases. |
| 供应商 | CST |
| 背景 | Platelet derived growth factor (PDGF) family proteins form dimers (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind receptor tyrosine kinases PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ) in a specific pattern. PDGFRβ homodimers bind PDGF BB and DD homodimers and the PDGF AB heterodimer. Heteromeric receptor PDGF α/β binds PDGF B, C, and D homodimers and the PDGF AB heterodimer (1). Ligand binding induces PDGF receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. Activated PDGF receptors initiate signaling pathways that control cell growth, actin reorganization, migration, and differentiation (2). PDGFRβ kinase-insert region residue Tyr751 forms the PI3 kinase docking site, and phosphorylation of PDGFRβ at this site inhibits the association between the SH2 domain of the PI3 kinase p85 subunit and PDGFRβ (3,4). |
| 存放说明 | -20C |
| 参考文献 | 1 . Deuel, T.F. et al. (1988) Biofactors 1, 213-7. 2 . Qu, C.K. (2000) Cell Res 10, 279-88. 3 . Franke, T.F. et al. (1997) Cell 88, 435-7. 4 . Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 5 . Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25. 6 . Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602. 7 . Baccarini, M. (2005) FEBS Lett 579, 3271-7. 8 . Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 9 . Franke, T.F. et al. (1995) Cell 81, 727-36. 10 . Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39. 11 . Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9. 12 . Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 13 . Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 14 . Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38. 15 . Lu, W. et al. (2001) Mol Cell 8, 759-69. 16 . Ramalingam, K. et al. (1995) Bioorg Med Chem 3, 1263-72. 17 . George, D. (2001) Semin Oncol 28, 27-33. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody (upper) or control SHP-2 Antibody #3752 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human glioblastoma using PDGF Receptor β (28E1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PDGF Receptor β (28E1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded U-87MG cells, showing membrane localization, using PDGF Receptor β (28E1) Rabbit mAb. | |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb. | |
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb in the presence of control peptide (left) or #1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific) (right). | |
Western blot analysis of extracts from various cell lines, using PDGF Receptor β (28E1) Rabbit mAb. | |
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (upper), or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower). | |
Confocal immunofluorescent analysis of NIH/3T3 cells, serum-starved (left) or PDGF-treated (right), using PDGF Receptor beta (28E1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower). | |
Confocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Akt (pan) (C67E7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
