| 货号 | 4750T |
| 目标/特异性 | Phospho-PAK2 (Ser20) Antibody, PAK1 Antibody, PAK2 (C17A10) Rabbit mAb, PAK3 Antibody and PAK1/2/3 Antibody detect endogenous levels of their target protein(s) and do not cross-react with other PAK family members. Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197), Phospho-PAK1 (Ser144)/PAK2 (Ser142), and Phospho-PAK1 (Thr423)/PAK2 (Thr402) antibodies, which may also detect Ser200/205, Ser139, and Thr421 of phosphorylated PAK3, respectively. Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody cross-reacts with phospho-Mst1 (Thr183) or phospho-Mst2 (Thr180). |
| 供应商 | CST |
| 背景 | The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation of Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation of Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). |
| 存放说明 | -20C |
| 参考文献 | 1 . Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-862. 2 . Daniels, R.H. et al. (1998) EMBO J. 17, 754-764. 3 . King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-41209. 4 . Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-1143. 5 . Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8028. 6 . Lei, M. et al. (2000) Cell 102, 387-397. 7 . Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-17353. 8 . Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-3917. |
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1/2/3 Antibody. | |
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody. | |
Immunoprecipitation of PAK1 from C6 and SH-SY5Y cells followed by Western blot analysis, using PAK1 Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using PAK1 Antibody. | |
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP (1 µM ) for the indicated times, using Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from guinea pig neutrophils stimulated with 1 µM fMLP for indicated times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (Provided by Drs. Qian Zhan and John Badwey, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts.) | |
Western blot analysis of extracts from COS cells cotransfected with activated cdc42 and GST-PAK2 (lane 1), transfected with GST-PAK2 alone (lane 2) or activated cdc42 alone (lane 3), using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower). This antibody detects GST-PAK2 signal only when it is cotransfected with activated cdc42, indicating that this antibody is phospho-specific. | |
Western blot analysis of extracts from HeLa cells treated with 0.4 M sorbitol for indicted times, using Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody (upper) or PAK2 antibody (lower). | |
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for indicated times, using Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody (upper) and PAK1/2/3 Antibody #2604 (lower). | |
Western blot analysis of extracts from guinea pig neutrophils stimulated with fMLP for the indicated times, using Phospho-PAK2 (Ser20) Antibody (upper) or PAK1/2/3 Antibody #2604 (lower). | |
Western blot analysis of mouse brain extract and recombinant GST-PAK3, using PAK3 Antibody. | |
Immunoprecipitation of PAK3 from mouse brain extract, using PAK3 Antibody, followed by Western blot analysis using PAK3 Antibody (left), PAK1 Antibody #2602 (middle) or PAK2 Antibody #2608 (right). | |
Western blot analysis of extracts from 293, C2C12 and COS cells using PAK2 (C17A10) Rabbit mAb. | |
Western blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100 nM) for 20 minutes, using: Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601; PAK1 Antibody #2602; PAK 1/2/3 Antibody #2604; Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605; Phopsho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606; Phospho-PAK2 (Ser20) Antibody #2607; PAK3 Antibody #2609; and PAK2 Rabbit mAb #2615.免疫印迹（Western Blot）分析未处理或insulin(100 nM)处理20分钟的C2C12细胞，分别使用Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601; PAK1 Antibody #2602; PAK 1/2/3 Antibody #2604; Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605; Phopsho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606; Phospho-PAK2 (Ser20) Antibody #2607; PAK3 Antibody #2609和PAK2 Rabbit mAb #2615. |
