Protein Folding and Stability Antibody Sampler Kit【科瑞奥博】

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Protein Folding and Stability Antibody Sampler Kit

货号： 4889T 基本售价： 6830.0 元规格： -

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概述
货号	4889T
描述	这一抗体小包装试剂盒是用于研究蛋白折叠和稳定性过程相关蛋白的经济适用组合，这一试剂盒包含有每个目标蛋白足够进行4次western blot检测的一抗和二抗。 Western Blotting
目标/特异性	Skp1 Antibody detects endogenous levels of total Skp1 protein. Skp2 (D3G5) XP® Rabbit mAb recognizes endogenous levels of total Skp2 protein. This antibody is predicted to cross-react with Skp2α and Skp2ß. ISG15 (22D2) Rabbit mAb detects endogenous levels of the uncleaved precursor form of ISG15 protein. This antibody does not recognize the activated (cleaved) or conjugated forms of ISG15. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and NEDD8. NEDD8 (19E3) Rabbit mAb detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15. Ubiquitin (P4D1) Mouse mAb detects ubiquitin, polyubiquitin and ubiquitinated proteins. UBC3 Antibody detects endogenous levels of total UBC3 and UBC3B protein. SUMO-1 Antibody detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD). SUMO-2/3 Antibody detects endogenous levels of SUMO-2/3, but does not cross-react with recombinant SUMO-1.

性能
供应商	CST
背景	小调节蛋白泛素往往通过共价方式连接到许多细胞蛋白质，这一标签介导标记蛋白进入蛋白酶体降解。泛素首先与活化酶E1形成硫酯键复合体而被激活，然后被转移到泛素载体蛋白E2，然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1)，泛素——蛋白酶体途径参与多种生物过程和疾病发生（2-3）。泛素样蛋白家族包含三个小泛素相关修饰蛋白SUMO-1，-2和-3，神经前体细胞表达发育下调蛋白8（NEDD8）和干扰素刺激的15kDa蛋白（ISG15）（4-6）。他们共价连接到靶蛋白是可逆的多步骤的过程，类似于蛋白泛素化过程。成熟的修饰分子与激活酶E1偶联，然后结合到E2，然后通过E3连接酶结合到靶蛋白（7-10）。泛素主要调节目标蛋白质的降解（8），而SUMO、NEDD8和ISG15调控一部分底物蛋白以调节其他各种生物过程（4，11-18）。泛素化过程中，E2和E3蛋白的不同组合和相互作用产生可变的底物特异性（4）。 UBC3和UBC3B都属于E2泛素载体蛋白（19，20）。 SCF（Skp1/CUL1/F-box）E3泛素连接酶蛋白复合体是由三种蛋白质组分，包括S期激酶相关蛋白1（SKP1），cullin同源1（CUL1）和Skp2的F-box蛋白（21 -23）。 Application References
存放说明	-20C
参考文献	1 . Ciechanover, A. (1998) EMBO J 17, 7151-60. 2 . Chiba, T. and Tanaka, K. (2004) Curr. Protein Pept. Sci. 5, 177-184. 3 . Ritchie, K.J. and Zhang, D.E. (2004) Semin. Cell Dev. Biol. 15, 237-246. 4 . Pagano, M. (2004) Mol Cell 14, 414-6. 5 . Reed, S.I. (2003) Nat Rev Mol Cell Biol 4, 855-64. 6 . Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem Sci 28, 321-8. 7 . Matunis, M.J. et al. (1996) J Cell Biol 135, 1457-70. 8 . Bernardi, R. et al. (2000) Oncogene 19, 2447-54. 9 . Osaka, F. et al. (1998) Genes Dev. 12, 2263-2268. 10 . Loeb, K.R. and Haas, A.L. (1992) J. Biol. Chem. 267, 7806-7813. 11 . Semplici, F. et al. (2002) Oncogene 21, 3978-87. 12 . Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-68. 13 . Zhao, C. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 10200-10205. 14 . Pagano, M. et al. (1995) Science 269, 682-5. 15 . Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21. 16 . Hamerman, J.A. et al. (2002) J. Immunol. 168, 2415-2423. 17 . Stickle, N.H. et al. (2004) Mol. Cell. Biol. 24, 3251-3261. 18 . Duprez, E. et al. (1999) J Cell Sci 112 ( Pt 3), 381-93. 19 . Xirodimas, D.P. et al. (2004) Cell 118, 83-97. 20 . Gostissa, M. et al. (1999) EMBO J 18, 6462-71. 21 . Rodriguez, M.S. et al. (1999) EMBO J 18, 6455-61. 22 . Desterro, J.M. et al. (1998) Mol Cell 2, 233-9. 23 . Yu, Z. K. et al. (1998) Proc Natl Acad Sci. 95, 11324-11329.

参考图片

Confocal immunofluorescent analysis of HeLa cells using SUMO-2/3 (18H8) Rabbit mAb (green). Actin filaments were labeled with DyLight® 554 Phalloidin #13054 (red).

Western blot analysis of extracts from various cell lines using Skp2 (D3G5) XP® Rabbit mAb #2652.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Skp2 (D3G5) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using Skp2 (D3G5) XP^® Rabbit mAb.

Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or shSkp2, using Skp2 (D3G5) XP^® Rabbit mAb (upper) and GAPDH (D16H11) XP^®Rabbit mAb #5174 (lower).

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human Skp2 (hSkp2-Myc/DDK, +), using Skp2 (D3G5) XP^®Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Skp2 (D3G5) XP^® Rabbit mAb.

Western blot analysis of extracts from various cell lines using Skp2 (D3G5) XP^®Rabbit mAb.

Confocal immunofluorescent analyis of HeLa cells expressing either a non-targeting shRNA (shNT; left) or shSkp2 (right) using Skp2 (D3G5) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red) (Cell lines kindly provided by Dr. Wenyi Wei, Harvard Medical School.)

Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa), recombinant SUMO-2, recombinant SUMO-3, and extracts from C6 and PC12 cells, using SUMO-2/3 (18H8) Rabbit mAb #4971 (upper) and SUMO-1 Antibody #4930 (lower).

Western blot analysis of lysates from HeLa and A549 cells, untreated (-) or treated with IFN-α (1000 U/mL) (+) for 24 hours, using ISG15 (22D2) Rabbit mAb #2758.

Western blot analysis of lysates from HeLa, RAW, C6 and COS cells, using NEDD8 (19E3) Rabbit mAb #2754.

Western blot analysis of extracts from PC12 and NIH/3T3 cells using Skp1 Antibody #2156.

Western blot analysis of recombinant GST-SUMO-1 protein (38 kDa) and extracts from CAD, HeLa and PC-12 cells using SUMO-1 Antibody #4930.

Western blot analysis of extracts from Jurkat, CAD and C6 cells using UBC3 Antibody #4997.

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Protein Folding and Stability Antibody Sampler Kit

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Western Blotting

Application References