| 货号 | 8337T |
| 描述 | The Glycolysis Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform four western blot experiments with each primary antibody. |
| 目标/特异性 | GAPDH (D16H11) XP® Rabbit mAb, Hexokinase I (C35C4) Rabbit mAb, Hexokinase II (C64G5) Rabbit mAb, LDHA (C4B5) Rabbit mAb, PKM2 (D78A4) XP® Rabbit mAb, and PFKP Antibody all detect endogenous levels of their respective total proteins. Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total pyruvate dehydrogenase α1 subunit. PKM1/2 (C103A3) Rabbit mAb detects endogenous levels of total PKM (including M1 and M2) protein. |
| 供应商 | CST |
| 背景 | Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . WARBURG, O. (1956) Science 123, 309-14. 2 . Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95. 3 . Christofk, H.R. et al. (2008) Nature 452, 230-3. 4 . Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37. 5 . Morrison, N. et al. (1992) Hum Genet 89, 105-6. 6 . Semenza, G.L. (2007) Biochem J 405, 1-9. 7 . Strumiło, S. (2005) Acta Biochim Pol 52, 759-64. |
Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb #8164. | |
Western blot analysis of various cell extracts using Hexokinase II (C64G5) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). | |
Western blot analysis of extracts from various cell lines using PFKP (D4B2) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LDHA (C4B5) Rabbit mAb #3582. Wetern blot检测多种细胞系提取物，所用抗体为LDHA (C4B5) Rabbit mAb兔单抗 #3582。 | |
Western blot analysis of extracts from various cell lines using PKM1/2 (C103A3) Rabbit mAb #3190. Wetern blot检测多种细胞系提取物，所用抗体为 PKM1/2 (C103A3) Rabbit mAb 兔单抗#3190。 | |
Western blot analysis of extracts from various cell lines using Hexokinase I (C35C4) Rabbit mAb #2024. Wetern blot检测多种细胞系提取物，所用抗体为Hexokinase I (C35C4) Rabbit mAb 兔单抗#2024。 | |
Western blot analysis of extracts from various cell lines using Hexokinase II (C64G5) Rabbit mAb #2867. Wetern blot检测多种细胞系提取物，所用抗体为Hexokinase II (C64G5) Rabbit mAb 兔单抗#2867。 | |
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb #5174. Wetern blot检测多种细胞系提取物，所用抗体为GAPDH (D16H11) XP® Rabbit mAb 兔单抗#5174。 | |
Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205. Wetern blot检测多种细胞系提取物，所用抗体为Pyruvate Dehydrogenase (C54G1) Rabbit mAb 兔单抗#3205。 | |
Immunohistochemical analysis of paraffin-embedded mouse colon using GAPDH (D16H11) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP® Rabbit mAb #4053 (upper) or GAPDH (14C10) Rabbit mAb #2118. Wetern blot检测多种细胞系和小鼠骨骼肌提取物，所用抗体为PKM2 (D78A4) XP® Rabbit mAb兔单抗 #4053(上)和GAPDH (14C10) Rabbit mAb兔单抗 #2118.(下)。 | |
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (D16H11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of A204 cells using PKM2 (D78A4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (D16H11) XP® Rabbit mAb. |
