| 货号 | 8655T |
| 描述 | The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform four western blots per primary. |
| 目标/特异性 | Each antibody in the Wnt/β-Catenin Activated Targets Antibody Sampler Kit detects its respective target at endogenous levels. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter. c-Myc (D84C12) Rabbit mAb is not recommended for detection of Myc-tagged fusion proteins. TCF1/TCF7 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. |
| 供应商 | CST |
| 背景 | The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11). |
| 存放说明 | -20C |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Brantjes, H. et al. (2002) Biol Chem 383, 255-61. 5 . Zeilstra, J. et al. (2008) Cancer Res 68, 3655-61. 6 . Shtutman, M. et al. (1999) Proc Natl Acad Sci U S A 96, 5522-7. 7 . Mann, B. et al. (1999) Proc Natl Acad Sci U S A 96, 1603-8. 8 . Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6. 9 . Boon, E.M. et al. (2002) Cancer Res 62, 5126-8. 10 . Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8. 11 . Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205. |
Confocal immunofluorescent analysis of HCT-15 cells using LEF1 (C12A5) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from HCT15 cells, DLD1 cells, and mouse thymocytes using LEF1 (C12A5) Rabbit mAb #2230.使用LEF1 (C12A5) Rabbit mAb#2230对HCT15，DLD1和小鼠胸腺组织提取物进行western blot分析。 | |
Western blot analysis of extracts from Jurkat, RPMI 8226, and A20 cells using c-Myc (D84C12) XP® Rabbit mAb #5605.使用c-Myc (D84C12) XP®Rabbit mAb#5605对Jurkat, RPMI 8226,和A20细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from mouse intestine (MMP-7-positive), mouse colon (MMP-7-negative), and rat prostate (MMP-7-positive) tissues using MMP-7 (D4H5) XP® Rabbit mAb #3801.使用MMP-7 (D4H5) XP®Rabbit mAb#3801对小鼠小肠(MMP-7-positive)，小鼠结肠(MMP-7-negative)和大鼠前列腺(MMP-7-positive)组织提取物进行western blot分析。 | |
Western blot analysis of extracts from HT-29 (Met-positive), SK-BR-3 (Met-negative), and T-47D (Met-negative) cells using Met (D1C2) XP® Rabbit mAb #8198 (upper) or β-Actin Antibody #4967 (lower).使用Met (D1C2) XP®Rabbit mAb#8198 (上) 或 β-Actin#4967 (下)对HT-29 (Met-positive), SK-BR-3 (Met-negative),和T-47D (Met-negative)细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HCT29 cells, Colo201 cells, Jurkat cells, and mouse thymocytes using TCF1 (C63D9) Rabbit mAb #2203.使用TCF1 (C63D9)Rabbit mAb#2203对HCT29 ，Colo201，Jurkat细胞和小鼠胸腺组织提取物进行western blot分析。 | |
Western blot analysis of extracts from NIH/3T3 and SK-N-MC cells, untreated (-) or UV-treated (+), using c-Jun (60A8) Rabbit mAb #9165.使用c-Jun (60A8) Rabbit mAb #9165对没处理（-）或UV-处理（+）的NIH/3T3 和SK-N-MC细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa and PANC1 cells using CD44 (156-3C11) Mouse mAb #3570.使用CD44 (156-3C11)Mouse mAb#3570 对HeLa和PANC1细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa and 3T3 cells using Cyclin D1 (DCS6) Mouse mAb #2926.使用Cyclin D1 (DCS6)Mouse mAb #2926对HeLa和3T3细胞提取物进行western blot分析。 | |
Flow cytometric analysis of T-47D cells (blue) and HT-29 cells (green) using Met (D1C2) XP® Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with β-NGF #5221 (50ng/ml) for 2h and either 10 μl of c-Jun (60A8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP® Rabbit mAb. |
