| 货号 | 8674T |
| 描述 | The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform four western blots per primary. |
| 目标/特异性 | Each antibody in the Mitochondrial Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. SOD1 (71G8) Mouse mAb does not cross-react with other related proteins. HSP60 (D307) Antibody does not cross-react with other HSPs. |
| 供应商 | CST |
| 背景 | The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8). |
| 存放说明 | -20C |
| 参考文献 | 1 . Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159. 2 . Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466. 3 . Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283. 4 . Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59. 5 . Strumiło, S. (2005) Acta Biochim Pol 52, 759-64. 6 . Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31. 7 . Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9. 8 . Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12. |
Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb. | |
Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using HSP60 (D307) Antibody #4870. | |
对多个细胞系使用HSP60 (D307) Antibody #4870进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205. 对多个细胞系使用Pyruvate Dehydrogenase (C54G1) Rabbit mAb 兔单抗#3205进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using SDHA Antibody #5839. 对多个细胞系使用SDHA Antibody #5839进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using PHB1 Antibody #2426. 对多个细胞系使用PHB1 Antibody #2426进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using VDAC (D73D12) Rabbit mAb #4661. 对多个细胞系使用VDAC (D73D12) Rabbit mAb 兔单抗#4661进行Western blot分析。 |
