| 货号 | 8694T |
| 描述 | The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform four western blot experiments per primary antibody. |
| 目标/特异性 | Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members. |
| 供应商 | CST |
| 背景 | SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12). |
| 存放说明 | -20C |
| 参考文献 | 1 . Tachibana, M. et al. (2005) Genes Dev 19, 815-26. 2 . Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8. 3 . Nishioka, K. et al. (2002) Genes Dev. 16, 479-489. 4 . Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28. 5 . Kouskouti, A. et al. (2004) Mol. Cell 14, 175-182. 6 . Chuikov, S. et al. (2004) Nature 432, 353-360. 7 . Fang, J. et al. (2002) Curr Biol 12, 1086-99. 8 . Xiao, B. et al. (2005) Genes Dev 19, 1444-54. 9 . Couture, J.F. et al. (2005) Genes Dev 19, 1455-65. 10 . Brown, M.A. et al. (2006) Mol Cancer 5, 26. 11 . Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72. 12 . Huang, J. et al. (2006) Nature 444, 629-32. |
Western blot analysis of extracts from various cell lines using RBBP5 (D3I6P) Rabbit mAb. | |
Immunoprecipitation of RBBP5 from C2C12 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RBBP5 (D3I6P) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RBBP5 (D3I6P) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of RBBP5 (D3I6P) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1B Promoter Primers #11951, human CDKN2C promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb #2996.Western blot方法检测多种细胞系的提取物，使用的抗体为SET8 (C18B7) Rabbit mAb #2996。 | |
Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb #8729.Western blot方法检测多种细胞系的提取物，使用的抗体为SUV39H1 (D11B6) Rabbit mAb #8729。 | |
Western blot analysis of extracts from various cell lines using SET7/SET9 Antibody #2813.Western blot方法检测多种细胞系的提取物，使用的抗体为SET7/SET9 Antibody #2813。 | |
Western blot analysis of extracts from various cell lines using SMYD2 (D14H7) Rabbit mAb #9734.Western blot方法检测多种细胞系的提取物，使用的抗体为SMYD2 (D14H7) Rabbit mAb #9734。 | |
Western blot analysis of extracts from various cell lines using RBBP5 Antibody #8037.Western blot方法检测多种细胞系的提取物，使用的抗体为RBBP5 Antibody #8037 | |
Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb #5019.Western blot方法检测多种细胞系的提取物，使用的抗体为ASH2L (D93F6) XP® Rabbit mAb #5019。 | |
Western blot analysis of extracts from MCF-7 and 293 cells using ESET (C1C12) Rabbit mAb #2196.Western blot方法检测MCF-7和293细胞提取物，使用的抗体为ESET (C1C12) Rabbit mAb #2196. | |
Western blot analysis of extracts from HeLa and 293 cells using G9a/EHMT2 (C6H3) Rabbit mAb #3306.Western blot方法检测HeLa和293细胞提取物，使用的抗体为G9a/EHMT2 (C6H3) Rabbit mAb #3306。 | |
Western blot analysis of extracts from various cell lines using SMYD2 (D14H7) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using SUV39H1 (D11B6) Rabbit mAb. | |
Confocal imunofluorescent analysis of HeLa cells using ASH2L (D93F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb. |
