| 货号 | 9784T |
| 描述 | The Alzheimer Disease Antibody Sampler Kit provides an economical means of evaluating Alzheimer Disease-related signaling. The kit contains enough primary and secondary antibodies to perform four western blot experiments per primary antibody. |
| 目标/特异性 | β-Amyloid Antibody detects several isoforms of β-amyloid peptide (Aβ), such as Aβ-40, Aβ-42, etc., regardless of phosphorylation state. APP/β-Amyloid (NAB228) Mouse mAb detects endogenous levels of APP/β-Amyloid protein. Although this antibody recognizes both the phospho and non-phospho forms of the protein, it has been shown to prefer the phosphorylated form in some systems. BACE (D10E5) Rabbit mAb detects endogenous levels of total BACE protein. Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb detects endogenous levels of GSK-3α protein when phosphorylated at Ser21, and does not detect GSK-3β when phosphorylated at Ser9. GSK-3α/β (D75D3) XP® Rabbit mAb detects endogenous levels of total GSK-3α and GSK-3β protein. The antigen is 100% conserved between GSK-3α and GSK-3β in humans, monkeys, mice, and rats. Neurofilament-L (C28E10) Rabbit mAb detects endogenous levels of total Neurofilament-L protein. α-Synuclein (Syn204) Mouse mAb detects endogenous levels of total synuclein protein. This antibody detects recombinant α but not β-synuclein (Giasson, B.I. et al., 2000). Tau (Tau46) Mouse mAb detects endogenous levels of total tau protein, and also cross-reacts with MAP2 at 280 kDa. This antibody is predicted to detect all six isoforms of tau based on the amino acid sequence. |
| 供应商 | CST |
| 背景 | Alzheimer Disease (AD) is one of the most common neurodegenerative diseases worldwide. Clinically, it is characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles, which results in neuronal dysfunction and cell death. Central to this disease is the differential processing of the integral transmembrane glycoprotein Amyloid β (A4) precursor protein (APP) that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of AD patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (2). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (3). The extracellular deposition and accumulation of the released Aβ fragments and an α-synuclein fragment known as the non- Aβ fragment, form the main components of amyloid plaques in AD. GSK-3α regulates the production of Aβ peptides. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates Aβ production by specifically inhibiting the cleavage of APP by γ-secretase, thereby blocking accumulation of Aβ peptides in the brains of mice that overproduce APP (4). AD is also characterized by the presence of neurofibrillary tangles. These tangles are the result of hyperphosphorylation and oligomerization of the microtubule associated protein Tau and lead to apoptosis of the neuron. In particular, phosphorylation of Tau Ser396 by GSK-3 or CDK5 destabilizes microtubules in AD (5,6). Additionally, neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM) and heavy (NFH) subunits (7). Accumulation of neurofilaments are found in many human neurological disorders including AD (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2 . Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59. 3 . Walter, J. et al. (2001) J Biol Chem 276, 14634-41. 4 . Phiel, C.J. et al. (2003) Nature 423, 435-9. 5 . Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9. 6 . Bramblett, G.T. et al. (1993) Neuron 10, 1089-99. 7 . Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55. |
Confocal immunofluorescent analysis of paraffin-embedded human Alzheimers brain using APP/β-Amyloid (NAB228) Mouse mAb (green) and Phospho-p44/42 MAPK (Thr202/Tyr204) (197G2) Rabbit mAb #4377 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb. | |
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right). | |
Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb in the presence of control peptide (left) or Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide #1027 (right). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb. | |
免疫组化分析石蜡包埋的人乳腺癌切片，未处理（左）或经 λ-磷酸酶处理（右），使用的抗体是Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma , untreated (left) or lambda phosphatase treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb. | |
免疫组化分析石蜡包埋的LNCaP细胞，未处理（左）或 经LY294002 处理（右），使用的抗体是Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb. | |
Western blot 分析COS-7 细胞提取物，经 λ-磷酸酶 或PDGF 处理，使用的抗体是pho-GSK-3α (Ser21) (36E9) Rabbit mAb 兔单抗 (上图) 或 GSK-3α Antibody 兔多抗#9338 (下图)。 | |
Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower). |
