| 货号 | 9789T |
| 描述 | The Phospho-EGF Receptor Pathway Sampler Kit provides an economical means to evaluate the activation status of multiple members of the EGF receptor pathway, including phosphorylated EGF receptor, Stat5, c-Cbl, Shc, Gab1, PLCγ1, Akt and p44/42 MAPK. The kit includes enough primary and secondary antibodies to perform four western blot experiments.磷酸化EGFR通路小包装试剂盒为检测EGFR通路中的多个成员的激活状态提供了一种经济的方式，包括检测EGFR, Stat5, c-Cbl, Shc, Gab1, PLCγ1, Akt和p44/42 MAPK的磷酸化水平。该试剂盒包括足够进行4次WB试验的一抗和二抗。 |
| 目标/特异性 | Each antibody in the Phospho-EGF Receptor Pathway Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb may cross-react weakly with other tyrosine-phosphorylated proteins. Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb may cross-react with phosphorylated Gab2, Gab3, or activated receptor tyrosine kinases. Phospho-Shc (Tyr239/240) Antibody may cross-react with activated EGF receptor protein. |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 参考文献 | Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9. Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4. Hubbard, S.R. et al. (1994) Nature 372, 746-54. Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43. Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86. Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66. Rojas, M. et al. (1996) J Biol Chem 271, 27456-61. Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73. |
BxPC-3细胞提取物免疫印记分析，未处理或者EGF刺激，用磷酸化EGF Receptor (Tyr1068) (D7A5) XP® 兔单抗（上）及EGF Receptor抗体#2232（下）。 | |
Western blot analysis of extracts of BxPC-3 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb (upper) and EGF Receptor Antibody #2232 (lower). | |
Confocal immunofluorescent analysis of Drosophila egg chambers, untreated (top) or λ phosphatase-treated (bottom), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
COS细胞提取物免疫印记分析，未处理或者U0126 #9903 (10 µM1小时）或TPA #4174 (200 nM10分钟）处理，用磷酸化p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® 兔单抗#4370 及44/42 MAPK (Erk1/2) (3A7) 鼠单抗 #9107。 | |
Western blot analysis of extracts from COS cells, untreated or treated with either U0126 #9903 (10 µM for 1h) or TPA #4174 (200 nM for 10 m), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. | |
T47D细胞提取物免疫印记分析，未处理或者调蛋白刺激，用磷酸化Gab1 (Tyr627) (C32H2)兔单抗（上）或者Gab1抗体#3232（下）。 | |
冰冻SKOV3异种移植物免疫组化分析，用磷酸化Akt (Ser473) (D9E) XP®兔单抗。 | |
Western blot analysis of cell extracts from T47D cells, untreated or stimulated with heregulin, using Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb (upper) or Gab1 Antibody #3232 (lower). | |
Immunohistochemical analysis of frozen SKOV3 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent #8112 (left) to TBST/5% normal goat serum (right) using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060. | |
C2C12细胞共聚焦免疫荧光分析，U0126 # 9903处理（左，10μM 1小时）或TPA # 9905（右，200 nM15分钟），使用磷酸化p44 / 42MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® 兔单抗（绿色）。肌动蛋白丝已用Alexa Fluor® 555的鬼笔环肽# 8953（红色）标记。蓝伪色= DRAQ5®#4084（荧光DNA染料）。 | |
Jurkat细胞的流式细胞仪分析，U0126处理（蓝色）或者PMA处理（绿色），用磷酸化p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP®兔单抗。 | |
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb. | |
Jurkat细胞的流式细胞仪分析，未处理（绿色）或者 LY294002 #9901, wortmannin #9951和U0126 #9903 处理（蓝色），用磷酸化Akt (Ser473) (D9E) XP®兔单抗，对照使用非特异阴性对照抗体（红色）。 |
