| 货号 | 9946T |
| 描述 | 这个sampler kit 提供一个经济有效的研究Forkhead 信号通路的方法。试剂盒包含可以进行至少四次免疫印迹实验的一抗和二抗。 |
| 目标/特异性 | Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody detects endogenous levels of FoxO1/FoxO3a only when phosphorylated at Thr24 of FoxO1 or Thr32 of FoxO3a. The antibody cross-reacts with phosphorylated FoxO4 at Thr28, but not with FoxO1 family members phosphorylated at other sites. Phospho-FoxO1 (Ser256) Antibody detects endogenous levels of FoxO1 only when phosphorylated at Ser256. The antibody cross-reacts with Fox04 phosphorylated at Ser193. Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/Fox04 (Thr28) (4G6) Rabbit mAb detects endogenous levels of FoxO1 when phosphorylated at Thr24, of FoxO3a when phosphorylated at Thr32 or FoxO4 when phosphorylated at Thr28. FoxO1 (C29H4) Rabbit mAb detects endogenous levels of total FoxO1 protein. The antibody does not detect exogenously expressed family members FoxO3a or FoxO4. Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb detects endogenous levels of FoxO3a only when phosphorylated at Ser253. This antibody may cross-react with FoxO1 when overexpressed and phosphorylated at Ser251 or FoxO4 when overexpressed and phosphorylated at Ser197. Phospho-FoxO3a (Ser318/321) Antibody detects endogenous levels of FoxO3a only when phosphorylated at Ser318/321. The antibody is expected to cross-react with FoxO1 when phosphorylated at Ser322/325 based on the peptide sequence. FoxO3a (D19A7) Rabbit mAb recognizes endogenous levels of total FoxO3a protein. The FoxO4 Antibody detects endogenous levels of FoxO4. The antibody is sensitive to phosphorylation within the antigen and preferrentially detects unphosphorylated FoxO4. |
| 供应商 | CST |
| 背景 | Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生（1-3）。这个家族的三个成员(FoxO1、 FoxO4和FoxO3a)与线虫的DAF-16蛋白有序列相似性。DAF-16调节IGFR1、PI3K和 Akt 参与的信号通路。活化的forkhead家族成员作为肿瘤抑制因子阻断细胞周期并促进凋亡。任何FoxO成员的高表达均可激活细胞周期的抑制因子 p27 Kip1。Forkhead转录因子也参与 TGF-β调控的 p21 Cip1上调过程，这个过程能够被 PI3K负向调节 (7)。Forkhead转录因子被Akt磷酸化修饰苏氨酸（24位）和丝氨酸（256和319位）后失活，导致其出核并抑制转录因子的活性（8）。Forkhead转录因子活性也可以被去乙酰化酶sirtuin（SirT1）（9）抑制。 |
| 存放说明 | -20C |
| 参考文献 | Anderson, M.J. et al. (1998) Genomics 47, 187-99. Galili, N. et al. (1993) Nat Genet 5, 230-5. Borkhardt, A. et al. (1997) Oncogene 14, 195-202. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83. Guo, S. et al. (1999) J Biol Chem 274, 17184-92. Seoane, J. et al. (2004) Cell 117, 211-23. Arden, K.C. (2004) Mol Cell 14, 416-8. Yang, Y. et al. (2005) EMBO J 24, 1021-32. |
Flow cytometric analysis of MCF7 cells (green) and MRK-NU-1 cells (blue) using FoxO3a (D19A7) Rabbit mAb. Anti-rabbit (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. | |
Flow cytometric analysis of IGROV-1 (green) and Jurkat (blue) using FoxO1 (C29H4) Rabbit mAb. Anti-rabbit (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. | |
Western blot analysis of extracts from 293T cells, untreated (-) or treated with LY294002 #9901 (50 μM, overnight; +) and Wortmannin #9951 (1 μM, overnight; +), using Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb (upper) or FoxO3a (D19A7) Rabbit mAb #12829 (lower). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using FoxO3a (D19A7) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxO3a (D19A7) Rabbit mAb. | |
Confocal immunofluorescent analysis of PC-3 cells, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from 293T, MRK-nu-1 and Jurkat cells using FoxO3a (D19A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). | |
Immunohistochemical analysis of paraffin-embedded metastatic SKOV3 tumor in mouse lung using FoxO3a (D19A7) Rabbit mAb. Note nuclear staining in adjacent normal lung. | |
Immunohistochemical analysis of paraffin-embedded PC-3 (upper) and MRK-nu-1 (lower) cell pellets, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb. | |
Western blot analysis of extracts from Jurkat cells treated with either Calyculin A (#9902) or LY294002 (#9901) using Phospho-FoxO1 (Thr24)/(FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane in the absence (-) or presence (-) of calf intestinal phosphatase (CIP) after western transfer. | |
Western blot analysis of extracts from Jurkat cells treated with either Calyculin A (#9902) or LY294002 (#9901), NIH3T3 and COS-7 cells using Phospho-FoxO1 (Thr24)/(FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb to detect FoxO1, FoxO3a and FoxO4 when phosphorylated at the Thr24, Thr32, and Thr28 positions, respectively (left panel). Total FoxO1, FoxO3a and FoxO4 were detected using FoxO1 (C29H4) Rabbit mAb (#2880), FoxO3a (75D8) Rabbit mAb (#2497) and FoxO4 Antibody (#9472), respectively (right panel). | |
Western blot analysis of extracts from COS-7 cells transfected with FoxO4, GFP-FoxO1 or FoxO3a using Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599. western blot分析COS-7 细胞提取物，采用FoxO4, GFP-FoxO1 或 FoxO3a转染，所用抗体为Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599 | |
Western blot analysis of extracts from Jurkat and PC3 cells using FoxO3a (75D8) Rabbit mAb. western blot 分析Jurkat和PC3细胞提取物，所用抗体为FoxO3a (75D8) Rabbit mAb | |
Western blot analysis of extracts from IGROV-1 and COS-7 cells using FoxO1 (C29H4) Rabbit mAb #2880. western blot分析IGROV-1 和 COS-7提取物，所用抗体为FoxO1 (C29H4) Rabbit mAb #2880. | |
Immunohistochemical analysis of paraffin-embedded human colon using FoxO1 (C29H4) Rabbit mAb. |
