| 货号 | 9965T |
| 描述 | The HSP/Chaperone Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments with each antibody. |
| 目标/特异性 | HSP40 (C64B4) Rabbit mAb detects endogenous levels of total HSP40 protein. HSP60 (D307) Antibody detects endogenous levels of total HSP60 protein. HSP70 Antibody detects endogenous levels of total HSP70 protein (HSP70-Hom, HSP70-1). HSP90 (C45G5) Rabbit mAb detects endogenous levels of total HSP90 protein. HSF1 Antibody detects endogenous levels of total HSF1 protein. Calnexin (C5C9) Rabbit mAb detects endogenous levels of total calnexin protein. PDI (C81H6) Rabbit mAb detects endogenous levels of total PDI protein. BiP (C50B12) Rabbit mAb detects endogenous levels of total BiP protein. Each of these antibodies recognizes only its specific target. |
| 供应商 | CST |
| 背景 | HSP70 and HSP90 are molecular chaperones expressed constitutively under normal conditions to maintain protein homeostasis and are induced upon environmental stress (1). HSP70 and HSP90 interact with unfolded proteins to prevent irreversible aggregation and catalyze the refolding of their substrates in an ATP-dependent manner (1). HSP40 family proteins bind unfolded proteins and prevent their aggregation, and deliver unfolded protiens to HSP70 (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). HSP60 is also present in the cytosol of many cells and is induced by stress, inflammatory and immune responses, autoantibodies correlated with Alzheimers, coronary artery diseases, MS, and diabetes (4-7). Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones including calnexin, BiP and protein disulfide isomerase (PDI). Calenxin is a calcium-binding protein embedded in the ER membrane that retains newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (8,9). When protein folding is disturbed inside the ER, Bip synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists them to refold properly (10). PDI catalyzes the formation and isomerization of disulfide bonds required to reach a proteins native state (11). Heat shock gene transcription is regulated by a familly of heat shock factors (HSFs), transcriptional activators that bind to heat shock response elements (HSEs) located upstream of all heat shock genes (12). During attenuation from the heat shock response, HSF1 is repressed by direct binding of HSP70, HSP40/Hdj-1 and HSF binding protein 1 (HSBP1) (13). |
| 存放说明 | -20C |
| 参考文献 | 1 . Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816. 2 . Morimoto, R.I. (1998) Genes Dev 12, 3788-96. 3 . Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316. 4 . Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128. 5 . Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283. 6 . Williams, D.B. (2006) J Cell Sci 119, 615-23. 7 . Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32. 8 . Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938. 9 . Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58. 10 . Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128. 11 . Satyal, S.H. et al. (1998) Genes Dev 12, 1962-74. 12 . Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297. 13 . Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90. |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
HeLa cells were either untreated (left panel) or heat shocked (right panel) for 1h. Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and either 10 µl of HSF1 Antibody or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HSPA6 Promoter Primers #5551, human HSP70 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Calnexin (C5C9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb. | |
Immunohistochemical analysis of frozen SKOV-3 xenograft using BiP (C50B12) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HSP90 (C45G5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HSP40 (C64B4) Rabbit mAb. |
