| 货号 | 5132S |
| 描述 | PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CSTs product offering based upon superior performance in specified applications. |
| 供应商 | CST |
| 背景 | LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7). |
| 存放说明 | -20C |
| 参考文献 | Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9. Marignani, P.A. (2005) J Clin Pathol 58, 15-9. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43. Hardie, D.G. (2004) J Cell Sci 117, 5479-87. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32. |
Western blot analysis of extracts from various cell lines using LKB1 (D60C5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines, untreated or treated with TPA #9905 and UV, using Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb (upper) and LKB1 (D60C5) Rabbit mAb #3047 (lower). |
