| 货号 | 8220S |
| 供应商 | CST |
| 背景 | The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). |
| 存放说明 | -20C |
| 参考文献 | Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89. Gupta, S. et al. (1995) Science 267, 389-93. van Dam, H. et al. (1995) EMBO J 14, 1798-811. Livingstone, C. et al. (1995) EMBO J 14, 1785-97. |
Western blot analysis of extracts from 293 and NIH/3T3 cells, untreated or UV-treated, using ATF-2 (20F1) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma using ATF-2 (20F1) Rabbit mAb. | |
Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower). | |
Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb compared to a nonspecific negative control antibody (red). |
