| 货号 | 12692S |
| 反应种属 | Human/Mouse |
| 应用 | IHC-P |
| 目标/特异性 | SignalStain® Apoptosis (Cleaved Caspase-3) IHC Detection Kit detects endogenous levels of the activated caspase-3 large fragment (17/19 kDa) resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. This kit was developed for and is recommended for immunohistochemistry only. |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| 存放说明 | -20C |
| 参考文献 | Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. Nicholson, D.W. et al. (1995) Nature 376, 37-43. |
Immunohistochemical analysis of paraffin-embedded Kelly xenograft using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579 (left) or Rabbit (DA1E) mAb IgG XP®; SignalStain Isotype Control #12960 (right). 使用Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗#9579 (左) 或Rabbit (DA1E) mAb IgG XP®SignalStain® Isotype Control #12960 (右)对石蜡包埋的Kelly xenograft 进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded E14 mouse embryo using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579 (left) or Rabbit (DA1E) mAb IgG XP®; SignalStain Isotype Control #12960 (right). 使用Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗#9579 (左)或 Rabbit (DA1E) mAb IgG XP®SignalStain® Isotype Control #12960 (右)对石蜡包埋的E14小鼠胚胎进行免疫组化分析。 |
