| 货号 | 8109S |
| 描述 | The SignalStain® Proliferation/Apoptosis IHC Sampler Kit from Cell Signaling Technology allows the researcher to examine paraffin-embedded tissues or cells with antibodies that will detect cellular apoptosis or proliferation. Each antibody is validated for use in immunohistochemical assays using multiple approaches. Also included in the kit are control slides that can be used to verify the performance of each antibody and a primary antibody diluent. Please see table above for recommended diluent for each antibody in this kit. |
| 应用 | IHC-P |
| 目标/特异性 | Each antibody in the SignalStain® Proliferation/Apoptosis IHC Sampler Kit detects endogenous levels of its target protein and does not cross-react with any related proteins. |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments (2). Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (3). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle through inhibiting apoptosis and promoting cell division (4,5). Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (6). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 7). PCNA protein expression is a well-accepted marker of proliferation, and PCNA (PC10) Mouse mAb has been used to assess PCNA levels in hundreds of scientific studies. Histone H3, H2A, H2B, and H4 make up the core histones of the nucleosome. In response to various stimuli, the amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination, which affect the accessibility of chromatin to transcription factors (8). Phosphorylation of Histone H3 at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (9-11). |
| 存放说明 | 4C and -20C |
| 参考文献 | 1 . Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 2 . Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200. 3 . Kelman, Z. and ODonnell, M. (1995) Nucleic Acids Res 23, 3613-20. 4 . Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466. 5 . Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60. 6 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 7 . Li, F. et al. (1998) Nature 396, 580-584. 8 . Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. 9 . Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 10 . Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 11 . Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85. |
Immunohistochemical analysis of paraffin embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Phospho-Histone H3 (Ser10) Antibody, PCNA (PC10) Mouse mAb, Cleaved Caspase-3 (Asp175) Antibody and Survivin (71G4B7E) Rabbit mAb. Cell pellets are provided in the SignalSlide™ Cleaved Caspase-3 (Asp175) IHC Controls. 免疫组织化学分析石蜡包埋的Jurkat细胞沉淀，未经处理（左）或 etoposide (依托泊甙)处理（右），使用抗体为Phospho-Histone H3 (Ser10) Antibody、PCNA (PC10) Mouse mAb、Cleaved Caspase-3 (Asp175) Antibody和Survivin (71G4B7E) Rabbit mAb。细胞沉淀来自SignalSlide™ Cleaved Caspase-3 (Asp175) IHC Controls 。 | |
