| 货号 | 48243T |
| 目标/特异性 | NKX2.5 (E1Y8H) Rabbit mAb recognizes endogenous levels of total NKX2.5 protein. GATA-6 (D61E4) XP® Rabbit mAb recognizes endogenous levels of total GATA-6 protein. MEF2C (D80C1) XP® Rabbit mAb detects endogenous levels of total MEF2C protein. α-Actinin (D6F6) XP® Rabbit mAb recognizes endogenous levels of total α-actinin protein. Troponin I (D6F8) Rabbit mAb recognizes endogenous levels of total troponin I protein. Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein. Connexin 43 Antibody detects endogenous levels of total connexin 43. This antibody does not cross-react with other connexins. |
| 供应商 | CST |
| 背景 | Cardiogenesis is a complex developmental event involving numerous transcription factors. NKX2.5 is a member of the NKX homeobox transcription factor family, which plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Mutations in NKX2.5 are associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (1,2). GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (3-5). GATA-6 plays a critical role in endoderm development and knock out of GATA-6 is embryonic lethal due to defects in formation of the heart tube and a failure to develop extraembryonic endoderm (6). MEF2C is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (7,8). α-Actinin was first recognized as an actin cross-linking protein. The α-actinin protein interacts with a large number of proteins involved in signaling to the cytoskeleton, including those involved in cellular adhesion, migration, and immune cell targeting (9). The muscle isoforms 2 and 3 (ACTN2, ACTN3) localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (9). Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (10). Assays for measuring serum concentrations of cardiac muscle TnT (cTNT), as well as cTnI, have been reported for analyzing cardiac injury. Connexin 43 (Cx43) is a member of the large family of gap junction proteins, which assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (11,12). |
| 运输条件 | 0.75 |
| 存放说明 | -20C |
| 参考文献 | 1 . Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22. 2 . Otey, C.A. and Carpen, O. (2004) Cell Motil Cytoskeleton 58, 104-11. 3 . Ward, D.G. et al. (2002) J Biol Chem 277, 41795-801. 4 . Musil, L.S. et al. (1990) J Cell Biol 111, 2077-88. 5 . Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010. 6 . Martin, J.F. et al. (1994) Mol Cell Biol 14, 1647-56. 7 . Musil, L.S. and Goodenough, D.A. (1991) J Cell Biol 115, 1357-74. 8 . Benson, D.W. et al. (1999) J Clin Invest 104, 1567-73. 9 . Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15. 10 . Yu, Y.T. et al. (1992) Genes Dev 6, 1783-98. 11 . Reamon-Buettner, S.M. and Borlak, J. (2010) Hum Mutat 31, 1185-94. 12 . Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from untreated and PMA-treated C6, COS, HeLa and C2C12 cells using Connexin 43 Antibody. | |
Immunohistochemical analysis of paraffin-embedded mouse heart using Connexin 43 Antibody in the presence of control peptide (left) or antigen specific peptide (lright). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Connexin 43 Antibody. | |
Immunohistochemical analysis of paraffin-embedded human heart, using Connexin 43 Antibody. (High magnification, inset). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Connexin 43 Antibody. | |
Confocal immunofluorescent analysis of rat optic nerve and ciliary epithelium using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
See notes for additional testing details. | |
Immunohistochemical analysis of frozen mouse heart tissue, using Connexin 43 Antibody. | |
Confocal immunofluorescent analysis of confluent COS cells using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cells lines using MEF2C (D80C1) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of C2C12 cells, undifferentiated (left) or differentiated for 3 days (right), using MEF2C (D80C1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from human and rat heart and human skeletal muscle using Troponin T (Cardiac) Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human cardiac Troponin T (isoform 1) construct (+), using Troponin T (Cardiac) Antibody. |
