Procaspase Antibody Sampler Kit【科瑞奥博】

产品中心

当前位置：首页>产品中心

Procaspase Antibody Sampler Kit

货号： 12742T 基本售价： 6156.0 元规格： -

产品信息

概述
货号	12742T
目标/特异性	Each antibody in the Procaspase Antibody Sampler Kit detects endogenous levels of its respective target. Caspase-3 (8G10) Rabbit mAb detects full-length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at Asp175. Caspase-6 Antibody detects both full length caspase-6 (35 kDa) and the small subunit (15 kDa) of caspase-6 resulting from cleavage at Asp193. Caspase-7 (D2Q3L) Rabbit mAb detects both the full-length (35 kDa) and the large subunit (20 kDa) of caspase-7 resulting from cleavage at Asp198. Caspase-8 (1C12) Mouse mAb detects full length (57 kDa), the cleaved intermediate p43/p41, and the p18 fragment of caspase-8. Caspase-9 (C9) Antibody detects full-length caspase-9, as well as the large fragments resulting from cleavage at Asp315 and Asp330. PARP Antibody detects full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage at Asp214. Lamin A/C (4C11) Mouse mAb detects full-length lamin A and lamin C proteins, as well as the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) resulting from caspase cleavage. Caspase-9 (C9) Antibody detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments.

性能
供应商	CST
背景	Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 2, 8, 9, 10 and 12) are closely coupled to proapoptotic signals, which include the FasL, TNF-α, and DNA damage. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis (1,2).Caspase-8 (FLICE, Mch5, MACH) and Caspase-9 (ICE-LAP6, Mch6) are initiator caspases. CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activate caspase-8, leading to the release of the caspase-8 active fragments, p18 and p10 (3-6). Cytochrome c released from the mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (7-11).
存放说明	-20C
参考文献	1 . Muzio, M. et al. (1996) Cell 85, 817-27. 2 . Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. 3 . Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 4 . Budihardjo, I. et al. (1999) Annu Rev Cell Dev Biol 15, 269-90. 5 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 6 . Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 7 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 8 . Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9. 9 . Liu, X. et al. (1996) Cell 86, 147-157. 10 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 11 . Li, P. et al. (1997) Cell 91, 479-489. 12 . Tewari, M. et al. (1995) Cell 81, 801-809. 13 . Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724. 14 . Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. 15 . Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. 16 . Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106. 17 . Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57. 18 . Faleiro, L. et al. (1997) EMBO J 16, 2271-81. 19 . Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52. 20 . Duan, H. et al. (1996) J Biol Chem 271, 1621-5. 21 . Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8. 22 . Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23. 23 . Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84. 24 . Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93. 25 . Orth, K. et al. (1996) J Biol Chem 271, 16443-6. 26 . Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37. 27 . Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

参考图片

Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-8 (1C12) Mouse mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from NIH/3T3 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using PARP Antibody.

Western blot analysis of extracts from NIH/3T3 cells, untreated or staurosporine-treated (1 µM) and Jurkat cells, untreated or etoposide-treated (25 µM), using Caspase-6 Antibody.

Western blot analysis of HeLa (human) and NIH/3T3 (mouse) cell extracts, untreated and treated with 1 μM staurosporine (3 hr) in vivo, using Caspase-3 (8G10) Rabbit mAb.

Immunoprecipitation of cleaved caspase-3 from Jurkat cell extracts untreated (control) or treated with etoposide (25uM 5hrs) (apoptotic) using Caspase-3 (8G10) Rabbit mAb, and western probed with the same antibody.

Western blot analysis of extracts from Jurkat cells (human), L929 cells (mouse), and C6 cells (rat), untreated or treated with staurosporine or cytochrome c as indicated, using Caspase 9 (C9) Mouse mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® Caspase-3 siRNA I (+), using Caspase-3 (8G10) Rabbit mAb and p42 MAPK Antibody #9108. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the p42 MAPK Antibody is used to control for loading and specificity of caspase-3 siRNA.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® Caspase-3 siRNA II (+), using Caspase-3 (8G10) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Caspase-3 (8G10) Rabbit mAb confirms silencing of caspase-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of caspase-3 siRNA.

Western blot analysis of extracts from various cell lines using Lamin A/C (4C11) Mouse mAb.

Western blot analysis of extracts from THP-1 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hr), using Lamin A/C (4C11) Mouse mAb.

Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (green) and MAP2 Antibody #4542 (red).

Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).