| 货号 | 12879T |
| 目标/特异性 | Each activation state antibody recognizes the phosphorylated form of its target. All target residues are based on the sequence for mouse IRS-1, except Ser636/339 and Ser1101, which are based on the sequence for human IRS-1. IRS-1 (D23G12) Rabbit mAb recognizes total IRS-1 protein independent of its phosphorylation state. |
| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites, many of which are related to negative feedback loops activated during insulin signaling. Ser302 (human Ser307) of IRS-1 is regulated by FOX01 (5), IKKγ, and MYO1C (6). Ser307 (human Ser312) of IRS-1 is phosphorylated by JNK (7) and IKK (8). PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, and retinoic acid treatment (9-12). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 (human Ser616) and Ser632/635 (human Ser636/639), respectively (13,14). Phosphorylation of IRS-1 at Ser1097 (human Ser1101) is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (15).胰岛素受体底物1(IRS-1)是胰岛素受体激酶的一类主要底物(1)。 IRS-1包含多个酪氨酸磷酸化位点，可以作为含SH2结构域蛋白的停靠位点，这些蛋白调控着胰岛素的代谢和促生长功能(2-4)。IRS-1还含有超过30个潜在的丝氨酸/苏氨酸磷酸化位点，其中多个和胰岛素信号通路中的负反馈机制项链。IRS-1的Ser302(human Ser307)可以被FOX01 (5), IKKγ, 和 MYO1C (6)调控。IRS-1的Ser307(human Ser312)被JNK (7)和 IKK (8)磷酸化。胰岛素受体活化或其他刺激因素如TPA，IL-6和 视黄酸处理会引发PKC磷酸化小鼠IRS-1的Ser318(human Ser323) (9-12)。PKC和mTOR信号通路能够分别介导IRS-1的Ser612(human Ser616)和Ser632?635（human Ser636/639）磷酸化(13,14)。IRS-1的Ser1097 (human Ser1101)通过PKCθ介导磷酸化会导致细胞胰岛素通路的抑制，提示在某些肥胖模型中存在胰岛素抵抗的潜在机制(15)。 |
| 存放说明 | -20C |
| 参考文献 | 1 . del Rincón SV et al. (2004) Oncogene 23, 9269–79 2 . Sun, X.J. et al. (1991) Nature 352, 73-77. 3 . Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672. 4 . Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430. 5 . Wang, L.M. et al. (1993) Science 261, 1591-1594. 6 . Cao, Y. et al. (2006) J Biol Chem 281, 40242-51. 7 . Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189. 8 . Nakamori, Y. et al. (2006) J Cell Biol 173, 665-71. 9 . Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121. 10 . Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645. 11 . De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947. 12 . Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307. 13 . Greene, M.W. et al. (2004) Biochem J 378, 105-16. 14 . Weigert, C. et al. (2006) J Biol Chem 281, 7060-7. 15 . Moeschel, K. et al. (2004) J Biol Chem 279, 25157-63. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Immunoprecipitation of IRS-1 from differentiated C2C12 cell extracts, untreated or insulin-treated for the indicated times, using IRS-1 Antibody #2382, followed by Western blot with either Phospho-IRS-1 (Ser307) Antibody, or IRS-1 Antibody #2382. | |
Western blot analysis of IRS-1 (#2382) antibody-immunoprecipitated pellets from differentiated C2C12 cells, unstimulated, insulin-stimulated (100 nM for 15 min) or PMA-stimulated (200 mM for 30 min), using Phospho-IRS-1 (Ser1101) Antibody (upper) or IRS-1 Antibody #2382 (lower). | |
Western blot analysis of extracts from CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), untreated or insulin-treated (100 nM for 5 min), showing an increase in phospho-IRS-1 (Ser307) with insulin stimulation, using Phospho-IRS-1 (Ser307) Antibody. | |
Western blot analysis of insulin treated (100 nM, 5 min) MCF7 and C2C12 cells using Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb. | |
Western blot analysis of MCF-7 cell extracts, unstimulated and insulin-stimulated (100 nM for 5 min), using IRS-1 Antibody #2382 (left) and Phospho-IRS-1 (Ser307) Antibody (right). | |
Immunprecipitation of Phospho-IRS-1 from insulin treated MCF7 cell extracts using Phospho-IRS-1 (Ser1101) antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control. | |
Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated or treated with insulin, using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. | |
Western blot analysis of MCF7, C2C12 and RD cell lines using IRS-1 (D23G12) Rabbit mAb. | |
Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb (upper), or IRS-1 Antibody #3407 (lower). | |
Western blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100 nM, 5 min) using, Phospho-IRS-1 (Ser636/639) Antibody. | |
Western blot analysis of insulin treated (100 nM, 5 min) MCF7 and C2C12 cells using Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491. 使用Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491对胰岛素处理(100 nM, 5 min) MCF7和C2C12细胞进行western blot分析。 | |
Western blot analysis of extracts from CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), untreated (-) or insulin-treated (100 nM, 5 min; +), showing an increase in phospho-IRS-1 (Ser307) with insulin stimulation, using Phospho-IRS-1 (Ser307) Antibody #2381. 未处理(-)或胰岛素处理(100 nM, 5 min; +)的CHO IR/IRS-1细胞(转染了胰岛素受体和IRS-1)在胰岛素刺激时表现出增强的phospho-IRS-1 (Ser307)，使用Phospho-IRS-1 (Ser307) Antibody #2381进行western blot分析。 | |
Western blot analysis of extracts from serum-starved C2C12 cells, untreated (-) or insulin-treated (150 nM, 5 min; +), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610 (upper) or IRS-1 Antibody #3407 (lower). 未处理(-)或胰岛素处理(150 nM, 5 min; +)血清饥饿的C2C12细胞提取物，使用Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb 兔单抗#5610 (上) 或 IRS-1 Antibody #3407 (下)进行western blot分析。 | |
Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated (-) or treated with insulin (+), using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203 (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. 未处理(-)或胰岛素处理(+)的CHO IR/IRS-1细胞提取物，使用Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb 兔单抗#3203 (上和中)或 IRS-1 Antibody #2382 (下)进行western blot分析。 |
