| 货号 | 4749T |
| 描述 | The Integrin Antibody Sampler Kit provides an economical means to screen samples for α and β subunits of integrin molecules. The kit includes enough primary and secondary antibody to perform four Western blot experiments with each antibody. |
| 目标/特异性 | All antibodies in the Integrin Antibody Sampler Kit detect endogenous levels of their target α and β integrin subunits of the integrin dimer. None of the antibodies provided detect intact dimers or their activational state. Integrin α4 Antibody detects endogenous levels of integrin α4 mature protein (150kDa), α4 precursor protein (140kDa), as well as the 70 kDa cleaved C-terminal α4 fragment. |
| 供应商 | CST |
| 背景 | Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). |
| 存放说明 | -20C |
| 参考文献 | 1 . Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71. 2 . Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100. 3 . van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98. |
Flow cytometric analysis of A-204 cells (blue) and A-431 cells (green) using Integrin β4 (D8P6C) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from HT-29, LNCaP, and A-204 cells using Integrin β4 (D8P6C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Integrin β4 (D8P6C) XP® Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded HT-29 (left) and LNCaP (right) cell pellets using Integrin β4 (D8P6C) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human testes using Integrin β3 (D7X3P) XP® Rabbit mAb. | |
Western blot analysis of extracts from U-87 MG, HUVEC and C2C12 cell lines using Integrin β3 (D7X3P) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Integrin β3 (D7X3P) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Integrin β1 (D2E5) Rabbit mAb. | |
Flow cytometric analysis of Jurkat cells using Integrin α4 (D2E1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Integrin β1 (D2E5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human skin using Integrin β1 (D2E5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Integrin α4 (D2E1) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of Jurkat (positive; left) and K-562 (negative; right) cells using Integrin α4 (D2E1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
