| 货号 | 4759T |
| 描述 | The ER Protein Folding Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. |
| 目标/特异性 | Each antibody in the ER Protein Folding Antibody Sampler Kit detects endogenous levels of its target protein. |
| 供应商 | CST |
| 背景 | After their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins (3). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress proteins 72, 57 and 44 (ERp72, ERp57 and ERp44), constitute the PDI family (4,5,6). The ER also contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (7) with sequence homology to Hsp90 (8). BiP is another chaperone whose synthesis is increased when protein folding is disturbed. BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (9). |
| 存放说明 | -20C |
| 参考文献 | 1 . Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925. 2 . Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344. 3 . Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8. 4 . Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32. 5 . Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6. 6 . Jessop, C.E. et al. (2007) EMBO J 26, 28-40. 7 . Mazzarella, RA et al. (1990) J Biol Chem 265(2), 1094-101. 8 . Satoh, M et al. (2005) Cell Stress Chaperones 10(4), 278-84 9 . Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90. |
Flow cytometric analysis of A204 cells using BiP (C50B12) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb. | |
Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ERp44 (D17A6) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using ERp44 (D17A6) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using ERp44 (D17A6) XP® Rabbit mAb #3798. 对多种细胞系提取物使用ERp44 (D17A6) XP® Rabbit mAb兔单抗 #3798进行Western blot分析。 | |
Western blot analysis of extracts from various cell types using Ero1-Lα Antibody #3264. 对多个细胞系提取物使用Ero1-Lα Antibody #3264进行Western blot分析。 | |
Western blot analysis of extracts from various cell types using BiP (C50B12) Rabbit mAb #3177. 对多个细胞系提取物使用BiP (C50B12) Rabbit mAb 兔单抗#3177进行Western blot分析。 | |
Western blot analysis of extracts from various cell types using Grp94 Antibody #2104. 对多个细胞系提取物使用Grp94 Antibody #2104进行Western blot分析。 |
