| 货号 | 8340T |
| 描述 | The Jumonji Family Antibody Sampler Kit provides an economical means of evaluating total levels of Jumonji family proteins. The kit contains enough primary antibodies to perform four western blot experiments with each primary antibody. |
| 目标/特异性 | Each antibody in the Jumonji Family Antibody Sampler Kit detects endogenous levels of the respective target protein. |
| 供应商 | CST |
| 背景 | Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (1). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (1). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (1). The JMJD2 (Jumonji domain-containing protein 2) family, also known as JHDM3 (JmjC domain-containing histone demethylation protein 3) family, contains four members: JMJD2A/JHDM3A, JMJD2B/JHDM3B, JMJD2C/JHDM3C, and JMJD2D/JHDM3D. In addition to the JmjC domain, these proteins also contain JmjN, PHD and Tudor domains, the latter of which has been shown to bind to methylated histone H3 at Lys4 and Lys9, and methylated histone H4 at Lys20 (2,3). JMJD2 proteins have been shown to demethylate di- and tri-methyl histone H3 at Lys9 and Lys36, and function as both activators and repressors of transcription (4-9). JMJD2A, JMJD2C and JMJD2D function as coactivators of the androgen receptor in prostate tumor cells (5). In contrast, JMJD2A also associates with Rb and N-CoR corepressor complexes and is necessary for transcriptional repression of target genes (6,7). JMJD2B antagonizes histone H3 Lys9 tri-methylation at pericentric heterochromatin (8). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (10). The JARID (Jumonji/AT-rich interactive domain-containing protein) family contains four members: JARID1A (also RBP2 and RBBP2), JARID1B (also PLU-1), JARID1C (also SMCX), and JARID1D (also SMCY) (11). In addition to the JmjC domain, these proteins contain JmjN, BRIGHT, C5HC2 zinc-finger, and PHD domains, the latter of which binds to methylated histone H3 (Lys9) (11). All four JARID proteins demethylate di- and tri-methyl histone H3 Lys4; JARID1B also demethylates mono-methyl histone H3 Lys4 (12-14). JARID1A is a critical RB-interacting protein and is required for Polycomb-Repressive Complex 2 (PRC2)-mediated transcriptional repression during ES cell differentiation (15). A JARID1A-NUP98 gene fusion is associated with myeloid leukemia (16). JARID1B, which interacts with many proteins including c-Myc and HDAC4, may play a role in cell fate decisions by blocking terminal differentiation (17-19). JARID1B is over-expressed in many breast cancers and may act by repressing multiple tumor suppressor genes including BRCA1 and HOXA5 (20,21). JARID1C has been found in a complex with HDAC1, HDAC2, G9a, and REST, which binds to and represses REST target genes in non-neuronal cells (14). JARID1D is largely uncharacterized. PHF2 contains a JmjC domain, which may play a role in histone demethylation (1). |
| 存放说明 | -20C |
| 参考文献 | 1 . Chen, Z. et al. (2007) Proc Natl Acad Sci USA 104, 10818-23. 2 . Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27. 3 . Lee, J. et al. (2008) Nat Struct Mol Biol 15, 109-11. 4 . Whetstine, J.R. et al. (2006) Cell 125, 467-81. 5 . Shin, S. and Janknecht, R. (2007) Biochem Biophys Res Commun 359, 742-6. 6 . Gray, S.G. et al. (2005) J Biol Chem 280, 28507-18. 7 . Zhang, D. et al. (2005) Mol Cell Biol 25, 6404-14. 8 . Fodor, B.D. et al. (2006) Genes Dev 20, 1557-62. 9 . Cloos, P.A. et al. (2006) Nature 442, 307-11. 10 . Hu, Z. et al. (2001) Oncogene 20, 6946-54. 11 . Benevolenskaya, E.V. (2007) Biochem Cell Biol 85, 435-43. 12 . Christensen, J. et al. (2007) Cell 128, 1063-76. 13 . Yamane, K. et al. (2007) Mol Cell 25, 801-12. 14 . Tahiliani, M. et al. (2007) Nature 447, 601-5. 15 . Pasini, D. et al. (2008) Genes Dev 22, 1345-55. 16 . van Zutven, L.J. et al. (2006) Genes Chromosomes Cancer 45, 437-46. 17 . Secombe, J. et al. (2007) Genes Dev 21, 537-51. 18 . Barrett, A. et al. (2007) Int J Cancer 121, 265-75. 19 . Dey, B.K. et al. (2008) Mol Cell Biol 28, 5312-27. 20 . Barrett, A. et al. (2002) Int J Cancer 101, 581-8. 21 . Lu, P.J. et al. (1999) J Biol Chem 274, 15633-45. |
Western blot analysis of extracts from various cell lines using JMJD2B (D7E6) Rabbit mAb. | |
Confocal immunofluorescent analysis of LNCaP cells using JMJD2B (D7E6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from various cell lines using PHF2 (D45A2) Rabbit mAb #3497. 使用PHF2 (D45A2) Rabbit mAb #3497，免疫印迹(Western blot)分析不同细胞中PHF2 (D45A2)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using JMJD2B Antibody #2898. 使用JMJD2B Antibody #2898，免疫印迹(Western blot)分不同细胞中JMJD2B的蛋白水平。 | |
Western blot analysis of extracts from NCCIT cells using JMJD1B (6A1-1F5) Mouse mAb #5377. 使用JMJD1B (6A1-1F5) Mouse mAb #5377，免疫印迹(Western blot)分NCCIT细胞中JMJD1B (6A1-1F5)的蛋白水平。 | |
Western blot analysis of extracts from NCCIT and NTERA-2 cells using JMJD2A (C37E5) Rabbit mAb #5328. 使用JMJD2A (C37E5) Rabbit mAb #5328，免疫印迹(Western blot)分析NCCIT和NTERA-2细胞中JMJD2A (C37E5)的蛋白水平。 | |
Western blot analysis of extracts from NCCIT and F9 cells using JARID1C (D29B9) Rabbit mAb #5361. 使用JARID1C (D29B9) Rabbit mAb #5361，免疫印迹(Western blot)分析NCCIT和F9细胞中JARID1C (D29B9)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using JARID1A (D28B10) XP® Rabbit mAb #3876. 使用JARID1A (D28B10) XP® Rabbit mAb #3876，免疫印迹(Western blot)分析不同细胞中JARID1A (D28B10)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using JARID1B Antibody #3273. 使用JARID1B Antibody #3273，免疫印迹(Western blot)分析不同细胞中JARID1B的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JMJD2A (C37E5) Rabbit mAb. | |
Western blot analysis of extracts from NCCIT and NTERA-2 cell lines using JMJD2A (C37E5) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using JMJD1B (6A1-1F5) Mouse mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). | |
Western blot analysis of extracts from NCCIT and F9 cell lines using JARID1C (D29B9) Rabbit mAb. | |
Confocal immunofluorescent analysis of NTERA-2 cells using JMJD2A (C37E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from NCCIT cells using JMJD1B (6A1-1F5) Mouse mAb. |
