| 货号 | 8349T |
| 描述 | NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform four western blot experiments with each primary antibody. |
| 目标/特异性 | CHD3 Antibody, CHD4 Antibody, and MTA1 (D40D1) XP® Rabbit mAb recognize endogenous levels of respective total proteins. MBD3 Antibody recognizes endogenous levels of all three isoforms of the MBD3 protein (MBD3A, MBD3B, MBD3C) and does not cross-react with MBD2. RBAP46 (V415) Antibody recognizes endogenous levels of total RBAP46 protein and does not cross-react with the RBAP48 protein. HDAC1 (10E2) Mouse mAb and HDAC2 (3F3) Mouse mAb recognize endogenous levels of respective total proteins and do not cross-react with other HDAC proteins. |
| 供应商 | CST |
| 背景 | The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11). |
| 存放说明 | -20C |
| 参考文献 | 1 . Tong, J.K. et al. (1998) Nature 395, 917-21. 2 . Xue, Y. et al. (1998) Mol Cell 2, 851-61. 3 . Zhang, Y. et al. (1998) Cell 95, 279-89. 4 . Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7. 5 . Jones, P.L. et al. (1998) Nat Genet 19, 187-91. 6 . Fujita, N. et al. (2003) Cell 113, 207-19. 7 . Ng, H.H. et al. (1999) Nat Genet 23, 58-61. 8 . Zhang, Y. et al. (1999) Genes Dev 13, 1924-35. 9 . Zhang, Y. et al. (1997) Cell 89, 357-64. 10 . Verreault, A. et al. (1998) Curr Biol 8, 96-108. 11 . Wade, P.A. et al. (1999) Nat Genet 23, 62-6. |
Immunoprecipitation of CHD4 from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD4 (D8B12) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD4 (D8B12) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using MBD3 (N87) Antibody. | |
Western blot analysis of extracts from various cell lines using CHD4 (D8B12) Rabbit mAb. | |
Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D40D1) XP® Rabbit mAb #5647. 使用MTA1 (D40D1) XP® Rabbit mAb #5647，免疫印迹(Western blot)分析MCF7和A-204细胞中MTA1 (D40D1)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody #6882. 使用RBAP46 (V415) Antibody #6882，免疫印迹(Western blot)分析不同细胞中RBAP46 (V415)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb #5113. 使用HDAC2 (3F3) Mouse mAb #5113，免疫印迹(Western blot)分析不同细胞中HDAC2 (3F3)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using HDAC1 (10E2) Mouse mAb #5356. 使用HDAC1 (10E2) Mouse mAb #5356，免疫印迹(Western blot)分析不同细胞中HDAC1 (10E2)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using MBD3 Antibody #3896. 使用MBD3 Antibody #3896，免疫印迹(Western blot)分析不同细胞中MBD3的蛋白水平。 | |
Western blot analysis of extracts from 293 and NCCIT cells using CHD4 Antibody #4245. 使用CHD4 Antibody #4245，免疫印迹(Western blot)分析293和NCCIT细胞中CHD4的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded mouse 4T1A mouse syngeneic tumor using MTA1 (D40D1) XP® Rabbit mAb. | |
Western blot analysis of extracts from HeLa and Jurkat cells using CHD3 Antibody #4241. 使用CHD3 Antibody #4241，免疫印迹(Western blot)分析HeLa和Jurkat细胞中CHD3的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody. | |
Confocal immunofluorescent analysis of HeLa cells using RBAP46 (V415) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MTA1 (D40D1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb. |
