Cofilin Activation Antibody Sampler Kit【科瑞奥博】

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Cofilin Activation Antibody Sampler Kit

货号： 8354T 基本售价： 6156.0 元规格： -

产品信息

概述
货号	8354T
描述	The Cofilin Activation Antibody Sampler Kit provides an economical means to evaluate the presence and status of cofilin activation. The kit contains enough primary antibody to perform four western blot experiments per primary.
目标/特异性	Cofilin (D3F9) XP® Rabbit mAb detects endogenous levels of total cofilin1 protein. Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at Ser3. Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at Thr508 or 505. LIMK2 (8C11) Rabbit mAb detects endogenous levels of total LIMK2 protein and does not cross-react with LIMK1. TESK1 (D49D4) Rabbit mAb detects endogenous levels of total TESK1 protein. Chronophin/PDXP (C85E3) Rabbit mAb detects endogenous levels of total chronophin/PDXP protein. ROCK1 (C8F7) Rabbit mAb detects endogenous levels of total ROCK1 protein.

性能
供应商	CST
背景	Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
存放说明	-20C
参考文献	Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30. Condeelis, J. (2001) Trends Cell Biol 11, 288-93. Arber, S. et al. (1998) Nature 393, 805-9. Yang, N. et al. (1998) Nature 393, 809-12. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80. Okano, I. et al. (1995) J Biol Chem 270, 31321-30. Maekawa, M. et al. (1999) Science 285, 895-8. Edwards, D.C. et al. (1999) Nat Cell Biol 1, 253-9. Ohashi, K. et al. (2000) J Biol Chem 275, 3577-82. Sumi, T. et al. (2001) J Biol Chem 276, 670-6. Arber, S. et al. (1998) Nature 393, 805-9. Toshima, J. et al. (1995) J Biol Chem 270, 31331-7. Toshima, J. et al. (2001) Mol Biol Cell 12, 1131-45. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58. Toshima, J. et al. (2001) Biochem Biophys Res Commun 286, 566-73. LaLonde, D.P. et al. (2005) J Biol Chem 280, 21680-8. Tsumura, Y. et al. (2005) Biochem J 387, 627-37. Chandramouli, S. et al. (2008) J Biol Chem 283, 1679-91. Gohla, A. et al. (2005) Nat Cell Biol 7, 21-9. Nakagawa, O. et al. (1996) FEBS Lett 392, 189-93. Lee, J.H. et al. (2004) J Cell Biol 167, 327-37. Sebbagh, M. et al. (2005) J Exp Med 201, 465-71. Amano, M. et al. (1996) J Biol Chem 271, 20246-9. Kureishi, Y. et al. (1997) J Biol Chem 272, 12257-60. Totsukawa, G. et al. (2000) J Cell Biol 150, 797-806. Soosairajah, J. et al. (2005) EMBO J 24, 473-86. Kurita, S. et al. (2007) Genes Cells 12, 663-76. Kurita, S. et al. (2008) J Biol Chem 283, 32542-52.

参考图片

Immunoprecipitation of SSH1 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (lane 2) or SSH1 (E1K3W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using SSH1 (E1K3W) Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SSH1 protein (hSSH1-Myc/DDK; +), using SSH1 (E1K3W) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western blot analysis of extracts from various cell lines using SSH1 (E1K3W) Rabbit mAb.

Western blot analysis of COS cells, untreated or λ phosphatase-treated, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (upper) or Cofilin Antiobdy #3312 (lower).

Western blot analysis of extracts from various cell lines using Chronophin/PDXP (C85E3) Rabbit mAb #4686.使用Chronophin/PDXP (C85E3) Rabbit mAb #4686，免疫印迹（Western Blot）分析不同细胞中Chronophin/PDXP (C85E3)蛋白水平。

Western blot analysis of extracts from various cell lines using ROCK1 (C8F7) Rabbit mAb #4035.使用ROCK1 (C8F7) Rabbit mAb #4035，免疫印迹（Western Blot）分析不同细胞中ROCK1 (C8F7)蛋白水平。

Western blot analysis of extracts from various cell lines using TESK1 (D49D4) Rabbit mAb #4655.使用TESK1 (D49D4) Rabbit mAb #4655，免疫印迹（Western Blot）分析不同细胞中TESK1 (D49D4)蛋白水平。

Western blot analysis of extracts from Colo201 and Jurkat cells using LIMK2 (8C11) Rabbit mAb #3845.使用LIMK2 (8C11) Rabbit mAb #3845，免疫印迹（Western Blot）分析Colo201和Jurkat细胞中LIMK2 (8C11)蛋白水平。

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313.使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313，免疫印迹（Western Blot）分析不同细胞中Phospho-Cofilin (Ser3)蛋白水平。

Western blot analysis of extracts from various cell lines using Cofilin (D3F9) XP® Rabbit mAb #5175.使用Cofilin (D3F9) XP® Rabbit mAb #5175，免疫印迹（Western Blot）分析不同细胞中Cofilin (D3F9)蛋白水平。

Western blot analysis of extracts from various cell types using Cofilin (D3F9) XP^® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells using Cofilin (D3F9) XP^® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Western blot analysis of extracts from various cell types using ROCK1 (C8F7) Rabbit mAb.

Immunoprecipitation of ROCK1 from HeLa cell extracts using ROCK1 (C8F7) Rabbit mAb. Western blot detection was perfomed using the same antibody. Lane 1 is 5% input.

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.