| 货号 | 8606T |
| 描述 | The Actin Nucleation and Polymerization Antibody Sampler Kit provides an economical means to evaluate the presence and status of actin nucleation and polymerization. The kit contains enough primary antibody to perform four western blots per primary. |
| 目标/特异性 | ARP2 Antibody recognizes endogenous levels of total ARP2 protein. ARP3 Antibody recognizes endogenous levels of total ARP3 protein. This antibody does not cross-react with endogenous ARP2. Profilin-1 (C56B8) Rabbit mAb recognizes endogenous levels of total profilin-1 protein. This antibody may cross-react with profilin-2. Phospho-Rac1/cdc42 (Ser71) Antibody recognizes endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. This antibody may also detect phospho-RhoA (Ser73). Rac1/Cdc42 Antibody recognizes endogenous levels of total rac1 and cdc42 proteins. Based on sequence similarities, this antibody may also detect rac2 and rac3; this antibody does not cross-react with RhoA, RhoB, or RhoC. N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. This antibody does not cross-react wth the hematopietic protein, WASP. WAVE-2 (D2C8) XP® Rabbit mAb recognizes endogenous levels of total WAVE-2 protein. |
| 供应商 | CST |
| 背景 | Actin nucleation is the process of forming new actin filaments and is necessary to stimulate actin polymerization. Actin polymerization is vital for cell motility, cell division, and cell adhesion. Rac and Cdc42, members of the Rho-GTPase family, play key roles in actin dynamics, membrane trafficking, transcriptional regulation, cell growth, and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the intrinsic GTPase activity or Rac/Cdc42, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through binding of RhoGDI, a guanine dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by Cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate (4). Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP, but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (4). WAVE-2 is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in the brain (5). The highly conserved ARP2/3 complex is an important actin nucleation protein complex consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of existing actin filaments and formation of daughter filaments following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (6). Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting exchange of ADP for ATP (reviewed in 7). Profilins bind to proteins involved in the regulation of actin dynamics including paladin (8), dynamin-I (9), VASP (10), and N-WASP (11). |
| 存放说明 | -20C |
| 参考文献 | 1 . Millard, T.H. et al. (2004) Biochem J 380, 1-17. 2 . Witke, W. (2004) Trends Cell Biol 14, 461-9. 3 . Boukhelifa, M. et al. (2006) FEBS J 273, 26-33. 4 . Gareus, R. et al. (2006) J Biol Chem 281, 2803-11. 5 . Reinhard, M. et al. (1995) EMBO J 14, 1583-9. 6 . Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312. 7 . Suetsugu, S. et al. (1999) Biochem Biophys Res Commun 260, 296-302. 8 . Suetsugu, S. et al. (1998) EMBO J 17, 6516-26. 9 . Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85. 10 . Goley, E.D. and Welch, M.D. (2006) Nat Rev Mol Cell Biol 7, 713-26. 11 . Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80. |
Western blot analysis of extracts from various cell lines using WAVE-2 (D2C8) XP® Rabbit mAb #3659.使用WAVE-2 (D2C8) XP®Rabbit mAb #3659对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa, Jurkat, and COS cells using ARP3 Antibody #4738.使用ARP3抗体#4738对HeLa，Jurkat和COS细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using ARP2 Antibody #3128.使用ARP2 抗体 #3128对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Rac1/Cdc42 Antibody #4651.使用Rac1/Cdc42抗体#4651对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using N-WASP (30D10) Rabbit mAb #4848.使用N-WASP (30D10)Rabbit mAb#4848对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Profilin-1 (C56B8) Rabbit mAb #3246.使用Profilin-1 (C56B8)Rabbit mAb#3246对对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from A-431 cells, untreated or treated with EGF for the indicated times, using Phospho-Rac1/cdc42 (Ser71) Antibody #2461 (upper) or a total Rac1/cdc42 antibody (lower).使用Phospho-Rac1/cdc42 (Ser71) 抗体#2461 (上) 或 a total Rac1/cdc42 抗体(下)对未处理或经过EGF处理指定时间的A-431细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Rac1/Cdc42 Antibody. | |
Immunoprecipitation of WAVE-2 from HeLa cells using WAVE-2 (D2C8) XP® Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 5% input. | |
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or EGF-treated #9908 (right), using Wave-2 (D2C8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell types using WAVE-2 (D2C8) XP® Rabbit mAb. | |
Western blot analysis of extracts from HeLa, Jurkat and COS cells using ARP3 Antibody. | |
Western blot analysis of extracts from various cell types using Profilin-1 (C56B8) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using ARP2 Antibody. | |
Immunoprecipitation of N-WASP from MCF-7 cells, using N-WASP (30D10) Rabbit mAb. Western blot was performed using the same antibody. |
