| 货号 | 8665T |
| 描述 | The Rag and LAMTOR Antibody Sampler Kit is an economical means of detecting various Rag and LAMTOR proteins implicated within mTOR complex signaling. The kit contains enough primary and secondary antibody to perform four western blots with each antibody. |
| 目标/特异性 | LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb, LAMTOR2/ROBLD3 (D7C10) Rabbit mAb, and LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb recognize endogenous levels of each specific protein. In addition, each antibody may cross-react with other LAMTOR proteins. RagA (D8B5) Rabbit mAb recognizes endogenous levels of total RagA protein. This antibody may also recognize endogenous levels of total RagB protein. RagB Antibody recognizes endogenous levels of total RagB protein. RagC (D8H5) Rabbit mAb recognizes endogenous levels of total RagC protein. RagD Antibody recognizes endogenous levels of total RagD protein. |
| 供应商 | CST |
| 背景 | The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6). |
| 存放说明 | -20C |
| 参考文献 | Sancak, Y. et al. (2008) Science 320, 1496-501. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. Wullschleger, S. et al. (2006) Cell 124, 471-84. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61. Sancak, Y. et al. (2010) Cell 141, 290-303. |
Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb #8145. Western blot分析不同细胞系提取物，所用抗体为LAMTOR2/ROBLD3 (D7C10) Rabbit mAb兔单抗 #8145。 | |
Western blot analysis of extracts from various cell lines using RagC (D8H5) Rabbit mAb #9480. Western blot分析不同细胞系提取物，所用抗体为RagC (D8H5) Rabbit mAb 兔单抗#9480。 | |
Western blot analysis of extracts from mIMCD3, KNRK, and COS-7 cells using RagA (D8B5) Rabbit mAb #4357. Western blot 分析mIMCD3, KNRK, 和 COS-7 细胞，所用抗体为RagA (D8B5) Rabbit mAb 兔单抗#4357。 | |
Western blot analysis of extracts from various cell lines using LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb #8168. | |
Western blot分析不同细胞系提取物，所用抗体为LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb兔单抗 #8168。 | |
Western blot analysis of extracts from various cell lines using RagB (D18F3) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb #8975. western blot分析不同细胞系提取物，所用抗体为LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb 兔单抗#8975。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LAMTOR1/C11orf59 (D11H6) XP®Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP®Rabbit mAb. |
