| 货号 | 8696T |
| 描述 | The Angiogenesis Antibody Sampler Kit provides an economical means to investigate the angiogenic pathway downstream of VEGFR2. The kit contains enough primary antibody to perform four western blots per primary antibody. |
| 目标/特异性 | Each antibody in the Angiogenesis Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross-react with other Src family members or overexpressed phosphorylated RTKs. Phospho-VEGFR2 (Tyr1175) (19A10) Rabbit mAb may cross-react with VEGFR1. |
| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Meyer, M. et al. (1999) EMBO J 18, 363-74. 2 . Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. 3 . Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. 4 . Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4. 5 . Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. 6 . Takahashi, T. et al. (2001) EMBO J 20, 2768-78. 7 . Le Boeuf F et al. (2004) J Biol Chem 279, 39175–85 |
Confocal immunofluorescent analysis of HT1080 cells, starved overnight then treated with U0126 #9903 (10 uM, 2 h; left) or PDBu (Phorbol 12,13-Dibutyrate) #12808 (100 nM, 15 m; right) using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb. | |
Western blot analysis of extracts from COS and 293 cells, untreated (-) or UV-treated (+), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).Western blot方法检测COS细胞和293细胞提取物，细胞不处理（-）或用UV处理（+），使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (上图)或p38 MAPK Antibody #9212 (下图). | |
Western blot analysis of extracts from 293, NIH/3T3, and C6 cells, treated with λ phosphatase or TPA #4174 as indicated, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper), or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western blot方法检测293细胞, NIH/3T3细胞和C6细胞提取物，细胞用λ磷酸酶或TPA #4174按说明处理，使用的抗体为Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)或p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (下图). | |
Western blot analysis of extracts from serum-starved A-431 and A549 cells, untreated (-) or treated with hEGF #8916 (100 ng/ml, 15 min; +), or serum-starved NIH/3T3 cells, untreated (-) or treated with hPDGF-BB #8912 (50 ng/ml, 15 min; +), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713 (upper) or PLCγ1 (D9H10) XP® Rabbit mAb #5690 (lower).Western blot方法检测血清饥饿的A-431细胞、A549 细胞和NIH/3T3细胞提取物，A-431和A549细胞不处理（-）或用hEGF #8916 (100 ng/ml, 15 min; +)处理，NIH/3T3细胞不处理（-）或用hPDGF-BB #8912 (50 ng/ml, 15 min; +)处理，使用的抗体为Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713 (上图)或PLCγ1 (D9H10) XP® Rabbit mAb #5690 (下图). | |
Western blot analysis of extracts from A549 cells, untreated (-) or treated with calf intestinal phosphatase (CIP; +), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 (upper) or FAK Antibody #3285 (lower).Western blot方法检测A549 细胞提取物，细胞不处理（-）或用小牛肠磷酸酶处理(CIP; +)，使用的抗体为Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 (上图)或FAK Antibody #3285 (下图). | |
Western blot analysis of extracts NIH/3T3 cells, serum-starved (-) or treated with hPDGF-BB #8912 (100 ng/ml, 15 min; +), using Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 (upper) or Src (36D10) Rabbit mAb #2109 (lower).Western blot方法检测NIH/3T3细胞提取物，细胞经血清饥饿（-）或用hPDGF-BB #8912 (100 ng/ml, 15 min; +)处理，使用的抗体为Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 (上图)或Src (36D10) Rabbit mAb #2109 (下图). | |
Western blot analysis of extracts from PC-3 cells, untreated (-) or treated with LY294002 #9901 and wortmannin #9951 (+), and NIH/3T3 cells, serum-starved (-) or PDGF-treated (+), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).Western blot方法检测PC-3细胞和NIH/3T3细胞提取物，PC-3细胞不处理（-）或用LY294002 #9901和渥曼青霉素#9951处理(50 ng/ml, 2 min; +)处理，NIH/3T3细胞经血清饥饿（-）或PDGF处理（+），使用的抗体为Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图)或Akt (pan) (C67E7) Rabbit mAb #4691 (下图). | |
Western blot analysis of extracts from HUVE cells, untreated (-) or VEGF-treated (50 ng/ml, 2 min; +), using Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (upper) or VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower).Western blot方法检测HUVE细胞提取物，细胞不处理（-）或用VEGF处理(50 ng/ml, 2 min; +) ，使用的抗体为Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (上图)或VEGF Receptor 2 (55B11) Rabbit mAb #2479 (下图). | |
Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower). | |
Immunoprecipitation (IP)/Western blot analysis of extracts from serum-starved HeLa cells, untreated (-) or treated (+) with TPA #4174 (100 nM, 15 min) prior to lysis in SDS (lanes 1 and 2) or IP lysis buffer (lane 3, TPA-treated only). IP Lysates were then subjected to immunoprecipitation with Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (lane 4), PLCγ1 (D9H10) XP®Rabbit mAb #5690 (lane 5), or Normal Rabbit IgG #2729 (lane 6). The western blot was probed using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb. Lane 3 represents 10% input. | |
Western blot analysis of extracts from serum-starved A-431 and A549 cells, untreated (-) or treated (+) with hEGF #8916 (100 ng/mL, 15 min) or serum-starved NIH/3T3 cells, untreated (-) or treated (+) with hPDGF-BB #8912 (50 ng/mL, 15 min), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (upper) or PLCγ1 (D9H10) XP® Rabbit mAb #5690 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon (normal adjacent to tumor) using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb. | |
Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (blue) or TPA #4174 (green), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb. |
