| 货号 | 9787T |
| 描述 | The Sirtuin Antibody Sampler Kit provides an economical means of evaluating total levels of sirtuin proteins. The kit includes enough antibody to perform at least four western blot experiments with each primary antibody. |
| 目标/特异性 | Each antibody in the Sirtuin Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. |
| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The best characterized of these genes is Saccharomyces cerevisiaeSir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. SirT2, one of several mammalian Sir2 homologs, deacetylates α-tubulin on Lys40 and histone H4 on Lys16, and is implicated in cytoskeletal regulation and progression through mitosis (2,3). SirT2 protein is mainly cytoplasmic and is associated with microtubules and the HDAC6 tubulin deacetylase (2). |
| 存放说明 | -20C |
| 参考文献 | 1 . Guarente, L. (1999) Nat. Genet. 23, 281-285. 2 . North, B.J. et al. (2003) Mol. Cell 11, 437-444. 3 . Mostoslavsky, R. et al. (2006) Cell 124, 315-29. 4 . Vaquero, A. et al. (2006) Genes Dev. 20, 1256-1261. 5 . Liszt, G. et al. (2005) J Biol Chem 280, 21313-20. 6 . Scher, M.B. et al. (2007) Genes Dev 21, 920-8. 7 . Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35. 8 . Schwer, B. et al. (2002) J Cell Biol 158, 647-57. 9 . Onyango, P. et al. (2002) Proc Natl Acad Sci U S A 99, 13653-8. 10 . Schwer, B. et al. (2006) Proc Natl Acad Sci U S A 103, 10224-9. 11 . Newman, J.C. et al. (2012) J Biol Chem 287, 42436-43. 12 . He, W. et al. (2012) Trends Endocrinol Metab 23, 467-76. 13 . Voelter-Mahlknecht, S. et al. (2006) Int J Oncol 28, 899-908. 14 . Barber, M.F. et al. (2012) Nature 487, 114-8. |
Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb #12486.Western blot方法检测不同细胞系的提取物，使用的抗体为SirT6 (D8D12) Rabbit mAb #12486。 | |
Western blot analysis of extracts from various cell lines using SirT7 (D3K5A) Rabbit mAb #5360.Western blot方法检测不同细胞系的提取物，使用的抗体为SirT7 (D3K5A) Rabbit mAb #5360。 | |
Western blot analysis of mitochondrial extracts from SirT3 wild-type (WT) and knockout (KO) mouse liver, and of whole cell extracts from rat liver and human kidney, using SirT3 (D22A3) Rabbit mAb #5490 (upper) and AIF (D39D2) XP® Rabbit mAb #5318 (lower).Western blot方法检测 SirT3 wild-type (WT) 和 knockout (KO)小鼠肝细胞的线粒体提取物以及从大鼠肝和人肾细胞的全细胞提取物，使用的抗体为SirT3 (D22A3) Rabbit mAb #5490 (上图) 或AIF (D39D2) XP® Rabbit mAb #5318 (下图)。 | |
Western blot analysis of extracts from 293 cells expressing either nontargeting shRNA (293 shNT) or shRNA targeting SirT5 (293 shSirT5), and HCT 116, Raw 264.7, and H-4-II-E cells using SirT5 (D8C3) Rabbit mAb #8782 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). 293 shNT and 293 shSirT5 cells were kindly provided by David Lombard, University of Michigan. Western blot方法检测 表达nontargeting shRNA (293 shNT) 或shRNA targeting SirT5 (293 shSirT5)SirT3的293细胞以及HCT 116, Raw 264.7, H-4-II-E 细胞的提取物，使用的抗体为SirT5 (D8C3) Rabbit mAb #8782 (上图) 或β-Actin (D6A8) Rabbit mAb #8457 (下图)。293 shNT 和 293 shSirT5 细胞由David Lombard, University of Michigan惠赠. | |
Western blot analysis of extracts from various cell lines using SirT2 (D4O5O) Rabbit mAb #12650. Western blot方法检测不同细胞系的提取物，使用的抗体为 SirT2 (D4O5O) Rabbit mAb #12650。 | |
Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb #2134.Western blot方法检测不同细胞系的提取物，使用的抗体为SirT1 (D1D7) Rabbit mAb #2134。 | |
Western blot analysis of extracts from SirT2 wild-type (WT) and knockout (KO) mouse brain using SirT2 (D4O5O) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). SirT2 WT and KO mouse brain extracts were kindly provided by Dr. Gizem Donmez, Tufts University School of Medicine. | |
Western blot analysis of extracts from 293 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-SirT1 (Ser47) Antibody #2314 (upper) or SirT1 Antibody #2310 (lower).Western blot方法检测untreated (-) 或 λ phosphatase-treated (+)293 细胞的提取物，使用的抗体为Phospho-SirT1 (Ser47) Antibody #2314 (上图) 或 SirT1 Antibody #2310 (下图)。 | |
Western blot analysis of extracts from various cell lines using SirT2 (D4O5O) Rabbit mAb. | |
Immunoprecipitation of SirT6 from HCT 116 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 3) or SirT6 (D8D12) Rabbit mAb (lane 2). Lane 1 is 10% input. Western blot analysis was performed using SirT6 (D8D12) Rabbit mAb. | |
Western blot analysis of extracts from SirT6 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). SirT6 WT and KO MEF were kindly provided by Dr. David Lombard, University of Michigan. | |
Western blot analysis of extracts from HCT 116 cells, expressing either non-targeting shRNA (shNT) or shSirT6, using SirT6 (D8D12) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using SirT7 (D3K5A) Rabbit mAb. | |
Confocal immunofluorescent analysis of HCT 116 cells, expressing either non-targeting shRNA (shNT) (left) or shSirT6 (right), using SirT6 (D8D12) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from various cell lines using SirT6 (D8D12) Rabbit mAb. |
