| 货号 | 9656S |
| 描述 | The StemLight™ Pluripotency Anitbody Kit contains a panel of antibodies for the detection of proteins that are specifically expressed in human pluripotent cells. The kit can be used to track the pluripotent potential of human embryonic stem (ES) or induced pluripotent (iPS) cells. The loss of these markers indicates a loss of pluripotency or differentiation of the culture. The kit components are pre-optimized for parallel use in immunofluorescent analysis. Enough reagents are provided for 50 assays based on a working volume of 100 µl. |
| 目标/特异性 | Each antibody in the StemLight™ Pluripotency Antibody Kit detects endogenous levels of their respective pluripotency marker proteins. |
| 供应商 | CST |
| 背景 | Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells. Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated. SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neurominadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9). |
| 存放说明 | -20C |
| 参考文献 | Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76. Okita, K. et al. (2007) Nature 448, 313-7. Yu, J. et al. (2007) Science 318, 1917-20. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37. Draper, J.S. et al. (2002) J Anat 200, 249-58. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30. |
Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 14 days) (right panel), using Oct-4A (C30A3) Rabbit mAb (green, upper), Sox2 (D6D9) XP® Rabbit mAb (green, middle) and Nanog Antibody (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Note the loss of pluripotency markers (green) as cells differentiate along the neuronal lineage with retinoic acid treatment. Oct-4A (C30A3)兔单抗（绿色，上），Sox2 (D6D9) XP®兔单抗（绿色，中）和Nanog抗体（绿色，下）对未处理（左道）或维甲酸处理（10 µM all-trans RA 处理5天）的NTERA-2细胞进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。注意维甲酸处理导致神经细胞系分化过程中多潜能标记（绿色）会丢失。 | |
Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using Neurofilament-L (C28E10) Rabbit mAb #2837 (green, upper), and β3-Tubulin (TU-20) Mouse mAb #4466 (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the appearance of neuronal markers and structures as cells differentiate along the neuronal lineage with retinoic acid treatment.使用Neurofilament-L (C28E10)兔单抗#283（绿色，上）和β3-Tubulin (TU-20)鼠单抗#4466（绿色，下）对未处理（左道）或维甲酸处理（10 µM all-trans RA 处理5天）的NTERA-2细胞进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5#4084（DNA荧光染料）。注意维甲酸处理导致神经细胞系分化过程中神经元标记和结构的变化。 | |
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (green, upper), Sox2 (D6D9) XP® Rabbit mAb (green, middle) and Nanog (D73G4) XP® Rabbit mAb (green, lower). |
