| 货号 | 3019T |
| 描述 | The Met Signaling Antibody Sampler Kit provides an economical means to investigate Met signaling. The kit contains primary and secondary antibodies to perform four Western blots with each antibody. |
| 目标/特异性 | Each antibody in the Met Signaling Antibody Sampler Kit recognizes endogenous levels of its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Met (Tyr1234/1235) (D26) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated Src proteins in Western blot. Phospho-Met (Tyr1349) (130H2) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Met (Tyr1003) (13D11) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Gab1 (Tyr307) Antibody cross-reacts with phosphorylated Gab2 and potentially with phosphorylated Gab3. |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor), is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. Addition of a phosphate at cytoplasmic Tyr1003 is essential for ubiquitination and Met protein degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast cancers. Thus, Met is an attractive cancer therapeutic and diagnostic target (6). |
| 存放说明 | -20C |
| 参考文献 | 1 . Weidner, K.M. et al. (1993) Mol Immunol 30, 1003-11. 2 . Park, M. et al. (1986) Cell 45, 895-904. 3 . Bardelli, A. et al. (1997) Oncogene 15, 3103-11. 4 . Taher, T.E. et al. (2002) J Immunol 169, 3793-800. 5 . Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. 6 . Traxler, P. et al. (2001) Med Res Rev 21, 499-512. |
Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb #8198 (upper) or β-Actin Antibody #4967 (lower). | |
Flow cytometric analysis of MKN-45 cells, untreated (green) or treated with SU11274 (blue). | |
Immunohistochemical analysis using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb on SignalSlide™ Phospho-Met (1234/1235) IHC Controls #8118 [MKN45 cells, untreated (left) or SU11274-treated (right)]. | |
Confocal immunofluorescence analysis of MKN45 cells, untreated (left) or treated with SU11274 (1 μM, 3 hours; right), using Phospho-MET (Tyr1234/Tyr1235) (D26) XP® Rabbit mAb. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of T-47D cells (blue) and HT-29 cells (green) using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human metastatic lung carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded cell pellets, MKN-45 (left) and T-47D (right), using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human papillary renal cell carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HT-29 and T-47D cells using Met (D1C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of purified active Ron kinase using a Phospho-Ron (Ser1394) Antibody (A), a Phospho-Ron (Tyr1238) Antibody (B), Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb (C) and Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (D). This demonstrates that Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb does not cross-react with phospho-Ron by western analysis. | |
Immunohistochemical analysis of paraffin-embedded papillary renal cell carcinoma using Phospho-Met (Tyr1234/1225) (D26) XP® Rabbit mAb. | |
Western blot analysis of extracts from HT-29 (Met+), SK-BR-3 (Met-), and T-47D (Met-) cells using Met (D1C2) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). | |
Immunohistochemical analysis of frozen MKN-45 xenograft using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Met (D1C2) XP® Rabbit mAb. | |
Immunohistochemical analysis of frozen MKN45 xenograft using Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb. |
