| 货号 | 8338T |
| 描述 | The Phospho-Insulin/IGF Receptor Antibody Sampler Kit provides an economical means of evaluating total Insulin Receptor and IGF-I Receptor β protein levels as well as Insulin and IGF-I Receptor β phosphorylated at specific sites. The kit includes enough antibody to perform four western blot experiments with each primary antibody. |
| 目标/特异性 | Each antibody in the Phospho-Insulin/IGF Receptor Antibody Sampler Kit recognizes endogenous levels of the specific target protein, with activation state-specific antibodies recognizing target proteins only when phosphorylated at the indicated residues. IGF-I Receptor beta (111A9) Rabbit mAb detects endogenous levels of total IGF-I receptor beta protein and does not cross-react with insulin receptor. Phospho-IGF-I Receptor beta (Tyr1135) (DA7A8) Rabbit mAb detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1135. This antibody cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody detects endogenous levels of Tyr1131-phosphorylated IGF-I receptor and Tyr1146-phosphorylated insulin receptor. This antibody cross-reacts with activated PDGF, FGF and EGF receptors; ErbB2; and c-Met. Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, and does not cross-react with other related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb detects endogenous levels of IGF-I receptor β protein only when phosphorylated at Tyr980, and may cross-react with activated insulin receptors and FLT3. Insulin Receptor β (4B8) Rabbit mAb detects endogenous levels of total insulin receptor β and does not cross-react with IGF-I receptor β. |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 参考文献 | Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93. Baserga, R. (2000) Oncogene 19, 5574-81. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60. Baserga, R. (1999) Exp Cell Res 253, 1-6. White, M.F. et al. (1985) J Biol Chem 260, 9470-8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80. |
Western blot analysis of extracts from 293 (IGF-I receptor β+) and SK-UT-1 (IGF-I receptor β-) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower). | |
Flow cytometric analysis of SK-UT-1 (blue) and MCF7 (green) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of MCF7 (left) and SK-UT-1 (right) cells using IGF-I Receptor β (D23H3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Insulin Receptor β (4B8) Rabbit mAb #3025.Wetern blot检测多种细胞系提取物，所用抗体为Insulin Receptor β (4B8) Rabbit mAb #3025。 | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with IGF-I (+), using Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb #4568 (upper) and IGF-I Receptor β (111A9) Rabbit mAb #3018 (lower). Wetern blot检测MCF7细胞提取物，未处理或用 IGF-I (+)处理，所用抗体为Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb兔单抗 #4568 (上)和IGF-I Receptor β (111A9) Rabbit mAb 兔单抗#3018 (下)。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with IGF and serum starved (+), as well as H-4-II-E cells, untreated (-) or treated with insulin and serum starved (+), using Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024. Wetern blot检测Hela细胞提取物，未处理或用IGF-I和血清饥饿处理，以及H-4-II-E细胞提取物，未处理或用胰岛素和血清饥饿处理。所用抗体为Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb兔单抗 #3024. | |
Western blot analysis of extracts from 3T3-L1 adipocytes, untreated or insulin-treated (100 nM for the indicated times), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021 (upper) or control IR antibody (lower). Wetern blot检测3T3-L1脂肪细胞提取物，未处理或用胰岛素(指定时间100 nM)处理，所用抗体为Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021 (上)和对照 IR antibody(下)。 | |
Western blot analysis of extracts from MCF-7 cells, untreated (-) or treated with IGF-I (+), using Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb #3918 (upper) and IGF-I Receptor β Antibody #3027 (lower). Wetern blot检测MCF-7细胞提取物，未处理(-) 或用 IGF-I(+), 处理，所用抗体为Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb 兔单抗#3918 (上)和IGF-I Receptor β Antibody #3027 (下)。 | |
Western blot analysis of untreated and IGF-treated Hela cell extracts as well as untreated and insulin-treated H-4-II-E cell extracts using Phospho-IGF-I-Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151)(19H7) Rabbit mAb | |
Western blot analysis of extracts from MCF7 cells, untreated or stimulated with IGF-I, using Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb (upper) and IGF-I Receptor β Antibody #3027 (lower). | |
Immunprecipitation of Insulin Receptor beta from insulin treated mIMCD-3 cell extracts using Insulin Receptor beta antibody (Lane 1) Lane 2: No antibody control. Lane 3: Input control. | |
Western blot analysis of extracts from MCF-7 cells, untreated or IGF-I-treated, using Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb (upper) and IGF-I Receptor β (111A9) Rabbit mAb #3018 (lower). | |
β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb specifically binds to tyrosine phosphorylated IGF-1 and insulin receptors, but not other phosphorylated tyrosine kinases. Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β(Tyr1150/1151) (19H7) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). | |
Western blot analysis of extracts from 3T3-L1 adipocytes, untreated or insulin-treated (100 nM for the indicated times), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IR antibody (lower). | |
Western blot analysis of extracts from 293 cells, untreated or IGF-I-treated (100 nM for 2 minutes), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IGF-I Receptor antibody (lower). |
