| 货号 | 8614T |
| 描述 | The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform four western blots per primary antibody. |
| 目标/特异性 | Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) XP® Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins. |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6). |
| 存放说明 | -20C |
| 参考文献 | 1 . Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55. 2 . Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51. 3 . Moll, R. et al. (1982) Cell 31, 11-24. 4 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. 5 . Leader, M. et al. (1987) Histopathology 11, 63-72. 6 . Li, Z. et al. (1996) Dev Biol 175, 362-6. |
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb #8457.使用β-Actin (D6A8) Rabbit mAb #8457对多种细胞提取物进行western blot分析。 | |
Flow cytometric analysis of NIH/3T3 cells using β-Actin (D6A8) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Western blot analysis of extracts from C2C12 cells, and rat and human heart using Desmin (D93F5) XP® Rabbit mAb #5332.使用Rabbit mAb #5332对C2C12细胞，大鼠和人心脏提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Pan-Keratin (C11) Mouse mAb #4545.使用Pan-Keratin (C11)Mouse mAb #4545对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb #5741.使用Vimentin (D21H3) XP®Rabbit mAb #5741对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from COS-7, NIH/3T3, and PC12 cells using β-Tubulin (9F3) Rabbit mAb #2128.使用β-Tubulin (9F3)Rabbit mAb #2128对COS-7，NIH/3T3,和PC12细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb. | |
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) XP® Rabbit mAb #4543. As expected, A549 cells are negative for keratin 17.使用Keratin 17 (D73C7) XP®Rabbit mAb #4543对-431, HeLa, 和A549细胞提取物进行western blot分析。如期，A549细胞不表达keratin17. | |
Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). | |
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). |
