| 货号 | 8652T |
| 描述 | The AMPA Receptor Antibody Sampler Kit provides an economical means of evaluating the four subunits of AMPARs. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody. |
| 目标/特异性 | Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb recognizes endogenous levels of AMPA Receptor (GluR 1) protein only when phosphorylated at Ser845. While the literature refers to this residue as Ser845, it is Ser863 in the UniProt sequence P42261. Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody detects endogenous levels of GluR 2 protein only when phosphorylated at Tyr869, Tyr873, or Tyr876. It may also detect GluA 3 when phosphorylated at the conserved Tyr880, Tyr884, or Tyr887. These residues are not conserved in GluR 1 or GluR 4. AMPA Receptor (GluR 2) (D39F2) Rabbit mAb detects endogenous levels of total GluR 2 protein. The antibody is not predicted to recognize other AMPA receptor subunits (e.g. GluA1, GluA3, or GluA4) based on sequence homology of the antigen. AMPA Receptor 3 (GluA3) (D47E3) Rabbit mAb detects endogenous levels of total GluR 3 protein. The antibody is not predicted to detect other AMPA receptor subunits (e.g. GluA1, GluA2, or GluA4) based on sequence homology of the antigen. AMPA Receptor 4 (GluA4) (D41A11) XP® Rabbit mAb detects endogenous levels of total GluR 4 protein. AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb recognizes endogenous levels of total AMPA Receptor (GluA1) protein. |
| 供应商 | CST |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2-containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). |
| 存放说明 | -20C |
| 参考文献 | Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97. |
Western blot analysis of extracts from mouse brain, rat brain, and rat prefrontal cortex tissues using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb. | |
Immunoprecipitation of AMPA Receptor (GluR 1) from mouse brain extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb. | |
Confocal immunofluorescent analysis of mouse hippocampus using AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from rat brain, either 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody #3921 (upper) and AMPA Receptor (GluR 2/3/4) Antibody #2460 (lower). | |
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb #8070. | |
Western blot analysis of extracts from mouse brain and rat brain using Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb #8084. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide. | |
Western blot analysis of extracts from mouse and rat brain using AMPA Receptor (GluR 2) (D39F2) Rabbit mAb #5306. | |
Western blot analysis of extracts from mouse and rat brain using AMPA Receptor (GluR 3) (D47E3) Rabbit mAb #4676. | |
Western blot analysis of extracts from rat and mouse brain using AMPA Receptor (GluR 2/3/4) Antibody #2460. | |
Western blot analysis of extracts from mouse brain and rat brain using Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide. | |
Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb. | |
Western blot analysis of extracts from mouse and rat brains using AMPA Receptor (GluR 2) (D39F2) Rabbit mAb. | |
Confocal immunofluorescent analysis of rat retina using AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from mouse and rat brains using AMPA Receptor (GluR 3) (D47E3) Rabbit mAb. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
