| 货号 | 8683T |
| 描述 | The Tight Junction Antibody Sampler Kit provides an economical means to evaluate the presence of a number of proteins involved in tight junctions. The kit contains enough primary antibodies to perform four western blot experiments per primary antibody. |
| 目标/特异性 | CD2AP Antibody recognizes endogenous levels of total CD2AP protein. Claudin-1 Antibody recognizes endogenous levels of total claudin-1 protein. Based on sequence similarity, Claudin-1 Antibody may cross-react with claudin-2 protein. Occludin (D15G7) Rabbit mAb recognizes endogenous levels of total occludin protein. ZO-1 (D7D12) Rabbit mAb recognizes endogenous levels of total ZO-1 protein. ZO-2 Antibody recognizes endogenous levels of total ZO-2 protein. ZO-3 (D57G7) XP® Rabbit mAb detects endogenous levels of total ZO-3 protein. |
| 供应商 | CST |
| 背景 | Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 4). ZO-1 and ZO-2 are required for tight junction formation and function (5,6). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation (7-9). Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions (10). CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (11-13). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (14). CD2AP is also involved in the immunological synapse between CD2-expressing T cells and antigen presenting cells (15). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (13). |
| 存放说明 | -20C |
| 参考文献 | 1 . Hewitt, K.J. et al. (2006) BMC Cancer 6, 186. 2 . Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 3 . Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66. 4 . Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28. 5 . Traweger, A. et al. (2003) J Biol Chem 278, 2692-700. 6 . Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11. 7 . Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36. 8 . Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47. 9 . Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36. 10 . Umeda, K. et al. (2006) Cell 126, 741-54. 11 . Ooshio, T. et al. (2010) J Biol Chem 285, 5003-12. 12 . Miyata, M. et al. (2009) J Cell Sci 122, 4319-29. 13 . Miyata, M. et al. (2009) J Biol Chem 284, 24595-609. 14 . Kirsch, K.H. et al. (1999) Proc Natl Acad Sci U S A 96, 6211-6. 15 . Kirsch, K.H. et al. (2001) J Biol Chem 276, 4957-63. 16 . Lynch, D.K. et al. (2003) J Biol Chem 278, 21805-13. 17 . Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-81. 18 . Hutchings, N.J. et al. (2003) J Biol Chem 278, 22396-403. |
Western blot analysis of extracts from various cell lines using Afadin (D1Y3Z) Rabbit mAb. | |
Immunoprecipitation of afadin from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Afadin (D1Y3Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Afadin (D1Y3Z) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human skin using Claudin-1 (D5H1D) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded cell pellets, A-431 (left) and MCF7 (right), using Claudin-1 (D5H1D) XP® Rabbit mAb. | |
Immunoprecipitation of claudin-1 from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Claudin-1 (D5H1D) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Claudin-1 (D5H1D) Rabbit mAb. | |
Western blot analysis of extracts from A431 and MCF7 cells using Claudin-1 (D5H1D) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using Afadin Antibody #6492.Western blot检测多种细胞提取物，采用的抗体是Afadin Antibody #6492。 | |
Western blot analysis of extracts from K562, A431, and COS cells using CD2AP Antibody #2135.Western blot检测K562, A431和COS细胞提取物，采用的抗体是CD2AP Antibody #2135。 | |
Western blot analysis of extracts from various cell lines using ZO-1 (D7D12) Rabbit mAb #8193.Western blot检测多种细胞提取物，采用的抗体是ZO-1 (D7D12) Rabbit mAb #8193。 | |
Western blot analysis of extracts from various cell lines using Afadin Antibody. | |
Western blot analysis of extracts from various cell lines using ZO-2 Antibody #2847.Western blot分析多种细胞提取物，采用的抗体是ZO-2 Antibody #2847。 | |
Western blot analysis of extracts from A-431 and ZR-75 cells using Claudin-1 Antibody #4933. Western blot检测 A-431和ZR-75细胞提取物，采用的抗体是Claudin-1 Antibody #4933。 | |
Western blot analysis of extracts from MCF-7, HT-29, and HCT-15 cells using ZO-3 (D57G7) XP® Rabbit mAb #3704.Western blot检测 MCF-7, HT-29和HCT-15细胞提取物，采用的抗体是ZO-3 (D57G7) XP® Rabbit mAb #3704。 |
