| 货号 | 9092T |
| 描述 | The StemLight™ iPS cell Reprogramming Antibody Kit contains a panel of antibodies for the detection of various proteins, combinations of which have been used to reprogram somatic cells to Induced Pluripotent Stem (iPS) cells. The kit can be used to track efficiency of expression of the reprogramming factors following transfection, viral transduction and other means of protein delivery. The kit components are pre-optimized for parallel use in immunofluorescent analysis at a standard dilution, but components are also validated for use in other applications --please refer to individual data sheet information for application specific recommendations. Enough reagents are provided for 160 immunofluorescent assays based on a working volume of 100 μl. |
| 目标/特异性 | Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Sox2 (D6D9) XP® Rabbit mAb detects endogenous levels of total Sox2 protein. Nanog (D73G4) XP® Rabbit mAb detects endogenous levels of total nanog protein. LIN28A (D84C11) XP® Rabbit mAb detects endogenous levels of total LIN28A protein. c-Myc (D84C12) XP® Rabbit mAb detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276 or #2278). KLF4 Antibody detects endogenous levels of total KLF4 protein by western blot, but for immunofluorescence it is only recommended for detection of exogenously expressed KLF4 protein. All components of this kit have been validated for reactivity to human protein, however some components also have additional species cross reactivities. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components. |
| 供应商 | CST |
| 背景 | Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells. Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated. SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neurominadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9). |
| 存放说明 | -20C |
| 参考文献 | Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76. Okita, K. et al. (2007) Nature 448, 313-7. Yu, J. et al. (2007) Science 318, 1917-20. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37. Draper, J.S. et al. (2002) J Anat 200, 249-58. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30. |
Confocal immunofluorescent analysis of lentiviral transduced human fibroblast cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, c-Myc (D84C12) XP® Rabbit mAb #5605, and KLF4 Antibody #4038 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Oct-4A (C30A3) 兔单抗#2840, Sox2 (D6D9) XP® 兔单抗#3579, c-Myc (D84C12) XP® 兔单抗#5605, 和KLF4抗体 #4038 (绿色)对慢病毒感染后的人成纤维细胞进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5#4084（DNA荧光染料）。 | |
Confocal immunofluorescent analysis of iPS cells using Oct-4A (C30A3) Rabbit mAb #2840, Sox2 (D6D9) XP® Rabbit mAb #3579, Nanog (D73G4) XP® Rabbit mAb #4903, c-Myc (D84C12) XP® Rabbit mAb #5605, and LIN28A (D84C11) XP® Rabbit mAb #3695 (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Oct-4A (C30A3) 兔单抗#2840, Sox2 (D6D9) XP® 兔单抗#3579, Nanog (D73G4) XP® 兔单抗 #4903, c-Myc (D84C12) XP® 兔单抗#5605, 和LIN28A (D84C11) XP® 兔单抗 #3695 (绿色)对iPS细胞进行激光共聚焦荧光分析。使用DY-554鬼笔环肽（红色）标记肌动蛋白丝。蓝色假色=DRAQ5#4084（DNA荧光染料）. | |
Flow cytometric analysis of HeLa cells (blue) and NTERA-2 cells (green) using Nanog (D73G4) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (D73G4) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP® Rabbit mAb. | |
Flow cytometric analysis of Raji cells using c-Myc (D84C12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus using Sox2 (D6D9) XP (TM) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® c-Myc siRNA I #6341 (right), using c-Myc (D84C12) XP® Rabbit mAb (green). Actin filaments have been labeled wth DY-554 phalloidin (red). | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® c-Myc siRNA I #6341, using c-Myc (D84C12) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using c-Myc (D84C12) XP® Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using KLF4 antibody. | |
Confocal immunofluorescent analysis of NTERA-2 cells using Lin28A (D84C11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using LIN28A (D84C11) XP® Rabbit mAb. | |
Western blot analysis of extracts of NTERA-2 and IGROV1 cells using LIN28A (D84C11) XP® Rabbit mAb. |
