| 货号 | 9384T |
| 描述 | The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. |
| 目标/特异性 | The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins. |
| 供应商 | CST |
| 背景 | Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6). |
| 存放说明 | -20C |
| 参考文献 | Moll, R. et al. (1982) Cell 31, 11-24. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66. Zatloukal, K. et al. (2004) J Pathol 204, 367-76. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85. |
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells using Keratin 8/18 (C51) Mouse mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. | |
Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Keratin 7 (D1E4) XP® Rabbit mAb. | |
Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb #4465. Note that MCF7 cells are negative for Keratin 7, as expected. | |
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Keratin 7 (D1E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected. | |
Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb #4558. As expected, the protein is absent in A-431 and HeLa cells.使用Keratin 19 (BA17) Mouse mAb #4558，免疫印迹（Western Blot）分析不同细胞中Keratin 19蛋白水平。正如期望，该蛋白在A-431和HeLa细胞中缺乏。 | |
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) XP® Rabbit mAb #4543. As expected, keratin 17 is absent in A549 cells.使用Keratin 17 (D73C7) XP® Rabbit mAb #4543，免疫印迹（Western Blot）分析A-431、HeLa和A549细胞中Keratin 17蛋白水平。正如预期的keratin 17蛋白在A549细胞中是缺乏的。 | |
Western blot analysis of extracts from various cell lines using Keratin 8/18 (C51) Mouse mAb #4546.使用Keratin 8/18 (C51) Mouse mAb #4546，免疫印迹（Western Blot）分析不同细胞中Keratin 8/18 (C51)蛋白水平。 | |
Western blot analysis of extracts from HeLa and A-431 cells using Keratin 18 (DC10) Mouse mAb #4548.使用Keratin 18 (DC10) Mouse mAb #4548，免疫印迹（Western Blot）分析HeLa和A-431细胞中Keratin 18蛋白水平。 | |
Western blot analysis of extracts from various cell lines using Pan-Keratin (C11) Mouse mAb #4545.使用Pan-Keratin (C11) Mouse mAb #4545，免疫印迹（Western Blot）分析不同细胞中Pan-Keratin蛋白水平。 | |
Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17. |
