| 货号 | 9788T |
| 描述 | The Polycomb Group Antibody Sampler Kit provides an economical means of evaluating total levels of Polycomb Group Proteins. The kit contains enough primary and secondary antibodies to perform four western mini-blot experiments. |
| 目标/特异性 | Each antibody in the Polycomb Group Antibody Sampler Kit detects endogenous levels of target proteins. |
| 供应商 | CST |
| 背景 | The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell-cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, Eed-Ezh2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinates histone H2A on Lys119 (6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of an additional catalytic subunit RING1B (also RING2 or RNF2) (7). PcG proteins play an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and are required for maintenance of adult hematopoietic and neural stem cells, as well as embryonic stem cells (3,4,8-10). |
| 存放说明 | -20C |
| 参考文献 | Boyer, L.A. et al. (2006) Nature 441, 349-53. Lee, T.I. et al. (2006) Cell 125, 301-13. Park, I.K. et al. (2003) Nature 423, 302-5. Molofsky, A.V. et al. (2003) Nature 425, 962-7. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67. Wang, H. et al. (2004) Nature 431, 873-8. Cao, R. et al. (2005) Mol Cell 20, 845-54. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7. Jacobs, J.J. et al. (1999) Nature 397, 164-8. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90. |
Flow cytometric analysis of human peripheral blood mononuclear cells untreated (left) and treated (right) with anti-human CD3 (10ug/ml, coated plates) and anti-human CD28 (5ug/ml) for 3 days at 37ºC using EZH2 (D2C9) XP® Rabbit mAb and co-stained with an anti-human CD3 antibody. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody. | |
Flow cytometric analysis of human whole blood cells using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (blue) and Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. Analysis was performed on cells in the lymphocyte gate. | |
Western blot analysis of extracts from various cell lines using Ring1A (D2P4D) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Ring1A (D2P4D) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb in the presence of non-methyl peptide (left) or K27 tri-methyl peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Bmi1 (D20B7) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Bmi1 (D20B7) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded 4T1a mouse syngeneic tumor using Ezh2 (D2C9) XP® Rabbit mAb. | |
Western blot analysis of various cell lines using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733. 使用Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733，免疫印迹(Western blot)分析不同细胞中Tri-Methyl-Histone H3 (Lys27)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using Bmi1 (D20B7) XP® Rabbit mAb #6964. 使用Bmi1 (D20B7) XP® Rabbit mAb #6964，免疫印迹(Western blot)分析不同细胞中Bmi1 (D20B7)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using RING1B (D22F2) XP® Rabbit mAb #5694. 使用RING1B (D22F2) XP® Rabbit mAb #5694，免疫印迹(Western blot)分析不同细胞中RING1B (D22F2)的蛋白水平。 | |
Western blot analysis of extracts of MOLT4 and LNCaP cells using Ring1A Antibody #2820. 使用Ring1A Antibody #2820，免疫印迹(Western blot)分析MOLT4和LNCaP细胞中Ring1A的蛋白水平。 | |
Western blot analysis of extracts from MCF7, Neuro-2a, and COS-7 cell lines using Ezh2 (D2C9) XP® Rabbit mAb #5246. 使用Ezh2 (D2C9) XP® Rabbit mAb #5246，免疫印迹(Western blot)分析MCF7、Neuro-2a和COS-7细胞中Ezh2 (D2C9)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using SUZ12 (D39F6) XP® Rabbit mAb #3737. 使用SUZ12 (D39F6) XP® Rabbit mAb #3737，免疫印迹(Western blot)分析不同细胞中SUZ12 (D39F6)的蛋白水平。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 5 µl of SUZ12 (D39F6) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one). |
