| 货号 | 9928T |
| 描述 | The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four Western blot experiments. |
| 目标/特异性 | The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments. Each antibody in the Histone Deacetylase (HDAC) Antibody Sampler Kit detects endogenous levels of its target and does not recognize other family members. |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | -20C |
| 参考文献 | Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18. |
Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, either untreated or treated for 1 h with TPA (0.2 uM) and ionomycin (0.33 uM), using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443. Phospho-specificity of the antibody was determined by treating cell extracts with lambda phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882. | |
Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb #7628. 使用HDAC4 (D15C3) Rabbit mAb #7628，免疫印迹(Western blot)分析不同细胞中HDAC4 (D15C3)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb #7558. 使用HDAC6 (D2E5) Rabbit mAb #7558，免疫印迹(Western blot)分析不同细胞中HDAC6 (D2E5)的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb. | |
Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb #3949. 使用HDAC3 (7G6C5) Mouse mAb #3949，免疫印迹(Western blot)分析不同细胞中HDAC3 (7G6C5)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb #5113. 使用HDAC2 (3F3) Mouse mAb #5113，免疫印迹(Western blot)分析不同细胞中HDAC2 (3F3)的蛋白水平。 | |
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb. | |
Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb #5356. 使用HDAC1 (10E2) Mouse mAb #5356，免疫印迹(Western blot)分析HeLa、NIH/3T3和H-4-II-E细胞中HDAC1 (10E2)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red). | |
Confocal immunofluorescent analysis of HeLa cells using HDAC1 (10E2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). | |
Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb. |
