mTOR Pathway Antibody Sampler Kit【科瑞奥博】

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mTOR Pathway Antibody Sampler Kit

货号： 9964T 基本售价： 5484.0 元规格： -

产品信息

概述
货号	9964T
描述	The mTOR Pathway Antibody Sampler Kit contains reagents to investigate the control of protein translation, cell growth, and proliferation through mTOR signaling within cells. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
目标/特异性	Each total antibody in the mTOR Pathway Sampler Kit recognizes only its specific target. Each phospho-specific antibody detects the intended target only when phosphorylated at the indicated site.

性能
供应商	CST
背景	The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
存放说明	-20C
参考文献	Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. Brown, E.J. et al. (1994) Nature 369, 756-8. Sabatini, D.M. et al. (1994) Cell 78, 35-43. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. Dennis, P.B. et al. (2001) Science 294, 1102-5. Fang, Y. et al. (2001) Science 294, 1942-5. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7. Hara, K. et al. (2002) Cell 110, 177-189. Kim, D.H. et al. (2002) Cell 110, 163-175. Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722. Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464. Oshiro, N. et al. (2004) Genes Cells 9, 359-366. Kim, D.H. et al. (2003) Mol. Cell 11, 895-904. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.

参考图片

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Confocal immunofluorescent analysis of mouse embryonic fibroblast (MEF) cells using mTOR (7C10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded mouse brain using mTOR (7C10) Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of mTOR siRNA.

Western blot analysis of extracts from various cell lines, using GβL (86B8) Rabbit mAb #3274.

Western blot 分析不同细胞类型提取物，所用抗体为GβL (86B8) Rabbit mAb兔单抗 #3274

Western blot analysis of extracts from 293 cells (starved for 30 hours), untreated or 20% FBS-treated for 30 minutes, using Phospho-mTOR (Ser2481) Antibody #2974.

Western blot 分析293细胞提取物（饥饿30小时），未处理组或20%FBS处理30分钟，所用抗体为Phospho-mTOR (Ser2481) Antibody #2974。

Western blot analysis of extracts from GβL wild-type (+/+) and knockout (-/-) mouse embryonic fibroblast cells using GβL (86B8) Rabbit mAb. (provided by David Sabatini Lab at the Whitehead Institute for Biomedical Research).

Western blot analysis of extracts from various cell types using Raptor (24C12) Rabbit mAb #2280.

western blot 分析不同细胞类型提取物，所用抗体为Raptor (24C12) Rabbit mAb 兔单抗#2280。

Western blot analysis of extracts from various cell types using Rictor (53A2) Rabbit mAb #2114.western blot 分析不同细胞类型提取物，所用抗体为Rictor (53A2) Rabbit mAb #2114

Western blot analysis of extracts from various cell types using mTOR (7C10) Rabbit mAb #2983.

Western blot 分析不同细胞类型提取物，所用抗体为mTOR (7C10) Rabbit mAb兔单抗 #2983

Western blot analysis of extracts from various cell lines, using GβL (86B8) Rabbit mAb.

Western blot analysis of extracts from various cell lines, using Raptor (24C12) Rabbit mAb.

Western blot analysis of extracts from various cell lines, using Rictor (53A2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using mTOR (7C10) Rabbit mAb.