| 货号 | 9941T |
| 描述 | The Pro-Survival Bcl-2 Family Antibody Sampler Kit provides an economical means to examine several members of the Bcl-2 family. The kit contains enough primary and secondary antibodies to perform four western blot experiments. |
| 目标/特异性 | Each antibody in the Pro-Survival Bcl-2 Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other Bcl-2 family members. Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of human Bcl-2 only when phosphorylated at Ser70. Phospho-Bcl-2 (Thr56) Antibody (Human Specific) detects endogenous levels of human Bcl-2 only when phosphorylated at Thr56. |
| 供应商 | CST |
| 背景 | The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (6). These phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and phosphorylation of Bcl-2 may be a marker for mitotic events (7,8). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (9). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced antiapoptotic functions (10). |
| 存放说明 | -20C |
| 参考文献 | Cory, S. et al. (2003) Oncogene 22, 8590-607. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. Maundrell, K. et al. (1997) J. Biol. Chem. 272, 25238-25242. Yamamoto, K. et al. (1999) Mol. Cell Biol. 19, 8469-8478. Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. |
Western blot analysis of extracts from various cell lines using Mcl-1 (D35A5) Rabbit mAb #5453. 使用Mcl-1 (D35A5) Rabbit mAb 兔单抗#5453对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence®BcL-xL siRNA I (+) or SignalSilence®Bcl-xL siRNA II #6363 (+), using Bcl-xL (54H6) Rabbit mAb #2764 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Bcl-xL (54H6) Rabbit mAb confirms silencing of Bcl-xL expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. | |
Western blot analysis of extracts from various cell lines using Mcl-1 (D35A5) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human or mouse Mcl-1 constructs, using Mcl-1 (D35A5) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Bcl-xL (54H6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of SH-SY5Y cells using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Bcl-2 (D55G8) Rabbit mAb (Human Specific). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bcl-2 siRNA I (+) or SignalSilence® Bcl-2 siRNA II #6516 (+), using Bcl-2 (50E3) Rabbit mAb and α-Tubulin (11H10) Rabbit mAb #2125. Bcl-2 (50E3) Rabbit mAb #2870 confirms silencing of Bcl-2 expression, while the α-tubulin (11H10) rabbit mAb is used to control for loading and specificity of Bcl-2 siRNA. | |
Western blot analysis of extracts from Jurkat and HeLa (human), COS (monkey), NIH/3T3 and L929 (mouse), and PC12 and C6 (rat) cells, using Bcl-xL (54H6) Rabbit mAb #2764. 使用Bcl-xL (54H6) Rabbit mAb 兔单抗#2764对Jurkat和HeLa (人)、COS (猴子)、NIH/3T3（小鼠）、L929 (小鼠)、PC12(大鼠) 和 C6 (大鼠) 细胞的提取物进行western blot分析。 | |
Western blot analysis of extracts from THP-1 cells, untreated or nocadazole-treated (1.0 µg/mL) overnight, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827 (upper), Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875 (middle) and Bcl-2 (50E3) Rabbit mAb #2870 (lower). 对未处理或nocadazole(1.0 µg/mL)处理过夜的THP-1细胞提取物，分别使用Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗#2827 (上)、Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875 (中) 和 Bcl-2 (50E3) Rabbit mAb 兔单抗#2870 (下)进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded 4TI syngeneic mouse tumor, using Bcl-xL (54H6) Rabbit mAb # 2764. | |
Western blot analysis of extracts from various cell types using Bcl-2 (50E3) Rabbit mAb. | |
Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content). | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1 μM, overnight) and with or without λ phosphatase, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (upper) or Bcl-2 #2876 (lower). | |
Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Bcl-xL (54H6) Rabbit mAb. |
