β-Catenin Antibody Sampler Kit【科瑞奥博】

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β-Catenin Antibody Sampler Kit

货号： 2951T 基本售价： 4822.0 元规格： -

产品信息

概述
货号	2951T
描述	The β-Catenin Antibody Sampler Kit provides an economical means of detecting total β-catenin as well as β-catenin phosphorlylated at various residues. The kit contains enough primary and secondary antibody to perform four Western blots with each antibody.
目标/特异性	Each antibody in this kit recognizes only its specific target and does not cross-react with other family members.

性能
供应商	CST
背景	β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
存放说明	-20C
参考文献	Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. Amit, S. et al. (2002) Genes Dev 16, 1066-76. Liu, C. et al. (2002) Cell 108, 837-47. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. Yost, C. et al. (1996) Genes Dev 10, 1443-54. Morin, P.J. et al. (1997) Science 275, 1787-90. Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976. Fang, D. et al. (2007) J. Biol. Chem. 282, 11221-11229. He, X.C. et al. (2007) Nat. Genet. 39, 189-198. Hino, S. et al. (2005) Mol. Cell Biol. 25, 9063-9072.

参考图片

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb #8480.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP^® Rabbit mAb.

Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP^® Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ HCT 116 cells and either 20 μl of β-Catenin (D10A8) XP^® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP^®Human Axin2 Intron 1 Primers #8973, SimpleChIP^®Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP^® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP^®Rabbit mAb.

Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP^® Rabbit mAb.

Flow cytometric analysis of NCI-H28 (blue) or HeLa (green) cells using β-Catenin (D10A8) XP^®Rabbit mAb.

Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP^®Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).

Western blot analysis of extracts from MKN-45 and SK-N-MC cells, untreated or treated with λ phosphatase for 1 hour or forskolin (FSK) for 30 minutes, using Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176(upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).使用Phospho-β-Catenin (Ser675) (D2F1) XP® 兔单抗#4176或-Catenin (6B3) 兔单抗 #9582 对未处理或者经过λ 磷酸酶处理1小时或福斯科林（FSK）处理30分钟的MKN-45 和SK-N-MC细胞提取物进行western blot分析。

Western blot analysis of extracts from SK-N-MC and NTERA-2 cells, untreated or λ phosphatase-treated, using