| 货号 | 3015T |
| 描述 | The Insulin Receptor Substrate Antibody Sampler Kit provides an economical means to investigate IRS-1 and IRS-2 signaling and phosphorylation within the cell. The kit contains enough antibody to perform four western blots with each primary antibody. |
| 目标/特异性 | Phospho-IRS-1 (Ser307) Antibody, Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb and Phospho-IRS-1 (Ser318) Antibody detect endogenous levels of IRS-1 only when phosphorylated at Ser307, Ser612 or Ser318, respectively. Note: Ser318 is the mouse residue; the corresponding human residue is Ser323. IRS-1 Antibody and IRS-2 Antibody detect endogenous levels of total IRS-1 or IRS-2, respectively. The Phospho-IRS-1 (Ser318) antibody cross reacts with a inducible nonspecific band at around 57 kDa. |
| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 参考文献 | Sun, X.J. et al. (1991) Nature 352, 73-77. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430. Wang, L.M. et al. (1993) Science 261, 1591-1594. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307. |
Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610 (upper), or IRS-1 Antibody #3407 (lower). 对血清饥饿的C2C12细胞（未处理或150nM胰岛素处理5分钟），使用Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb兔单抗 #5610 (upper)（上图）或IRS-1 Antibody #3407（下图）进行Western blot分析。 | |
Western blot analysis of MCF7, C2C12 and RD cell lines using IRS-1 (D23G12) Rabbit mAb #3407. 对MCF7、C2C12和RD细胞系使用IRS-1 (D23G12) Rabbit mAb兔单抗 #3407进行Western blot分析。 | |
Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb (upper), or IRS-1 Antibody #3407 (lower). | |
Western blot analysis of MCF7, C2C12 and RD cell lines using IRS-1 (D23G12) Rabbit mAb. | |
Western blot analysis of extracts from MCF-7 cells, unstimulated or insulin-stimulated (100 nM for 5 min), using IRS-1 Antibody #2382 (left) and Phospho-IRS-1 (Ser307) Antibody #2381 (right). 对MCF-7细胞（未处理或150nM胰岛素处理5分钟），使用IRS-1 Antibody #2382（左）或Phospho-IRS-1 (Ser307) Antibody #2381（右）进行Western blot分析。 | |
Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody #4502 (upper) or IRS-1 Antibody #2382 (lower). 对CHO IR/4PS细胞（转染胰岛素受体和IRS-2）或CHO IR/IRS-1细胞（转染胰岛素受体和IRS-1），使用 IRS-2 Antibody #4502（上图）或IRS-1 Antibody #2382（下图）进行Western blot分析，显示100nM胰岛素刺激5分钟总的蛋白水平没有变化。 | |
Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated or treated with insulin, using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. | |
Western blot analysis of MCF-7 cell extracts, unstimulated and insulin-stimulated (100 nM for 5 min), using IRS-1 Antibody #2382 (left) and Phospho-IRS-1 (Ser307) Antibody (right). | |
Western blot analysis of extracts from CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), untreated or insulin-treated (100 nM for 5 min), showing an increase in phospho-IRS-1 (Ser307) with insulin stimulation, using Phospho-IRS-1 (Ser307) Antibody. | |
Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody (top) or IRS-1 Antibody #2382 (bottom). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Immunoprecipitation of IRS-1 from differentiated C2C12 cell extracts, untreated or insulin-treated for the indicated times, using IRS-1 Antibody #2382, followed by Western blot with either Phospho-IRS-1 (Ser307) Antibody, or IRS-1 Antibody #2382. |
