Phospho-VEGF Receptor 2 Antibody Sampler Kit【科瑞奥博】

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Phospho-VEGF Receptor 2 Antibody Sampler Kit

货号： 12599T 基本售价： 4822.0 元规格： -

产品信息

概述
货号	12599T
目标/特异性	The phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb detects endogenous VEGF Receptor 2 at tyrosine 1175 and may cross-react with other tyrosine-phosphorylated receptor tyrosine kinases, including VEGFR1 and FLT3. The phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb

性能
供应商	CST
背景
存放说明	-20C
参考文献	Meyer, M. et al. (1999) EMBO J 18, 363-74. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7. Takahashi, T. et al. (2001) EMBO J 20, 2768-78. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

参考图片

Western blot analysis of extracts from CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr996) Antibody (upper) or VEGF receptor 2 antibody (lower).

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb specifically binds to phosphorylated VEGFR2, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). CKR/PAE cells (lanes 13 and 14) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain (5). CSF-1 stimulates phosphorylation of Tyr951 of intracellular VEGF receptor-2 domain (lane 13) , which was specifically detected by Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HUVEC cells, untreated (left) or VEGF-treated (right), using Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb.

Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb (upper) and VEGF Receptor 2 Antibody # 2472 (lower).

Western blot analysis of extracts from HUVEC, untreated or stimulated with VEGF #9943, using Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb (lower) #2479.

Western blot analysis of PAE/CKR cells, untreated or stimulated with CSF-1, using Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower). PAE/CKR cells express a chimeric receptor made up of human CSF-1 receptor extracellular domain and mouse VEGF receptor 2 transmembrane and intracellular domains.

Western blot analysis of extracts from HUVE cells, HeLa cells and mouse heart using VEGF Receptor 2 (D5B1) Rabbit mAb for VEGFR2 expression (upper) and β-Actin (D6A8) Rabbit mAb (#8457) for loading control. (The 80 kDa bands represent partial degradation product).

Confocal immunofluorescent analysis of rat pancreas using VEGF Receptor 2 (D5B1) Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human angiosarcoma using VEGF Receptor 2 (D5B1) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse liver using VEGF Receptor 2 (D5B1) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HCC827 xenograft using VEGFR2 (D5B1) Rabbit mAb.

Flow cytometric analysis of HeLa cells (blue) and HUVE cells (green) using VEGF Receptor 2 (D5B1) Rabbit mAb.

Western blot analysis of extracts from HUVE cells, HeLa cells, and mouse heart using VEGF Receptor 2 (D5B1) Rabbit mAb #9698 for VEGFR2 expression (upper) and β-Actin (D6A8) Rabbit mAb #8457 for loading control (The 80 kDa bands represent partial degradation product).

Western blot analysis of PAEC/CKR cells, untreated (-) or stimulated with CSF-1 (+), using Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817 (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower). PAEC/CKR cells express a chimeric receptor made up of human CSF-1 receptor extracellular domain and mouse VEGF receptor 2 transmembrane and intracellular domains.