| 货号 | 12718T |
| 目标/特异性 | Each antibody will detect endogenous total levels of their target protein. |
| 供应商 | CST |
| 背景 | Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation, and folding. The ER contains a pool of molecular chaperones to help proteins fold properly. Calnexin is a calcium-binding, ER membrane protein that ensures proper protein folding by retaining newly synthesized glycoproteins within the ER l (1-3). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (3). Many secretory proteins require the formation of intra- or inter-molecular disulfide bonds to reach their native conformation (4). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds during oxidative protein folding (5). The ER-protein Ero1 oxidizes PDI through disulfide exchange, which is followed by PDI-catalyzed disulfide bond formation in folding proteins (6). The ER stress protein 72 (ERp72) contains three thioredoxin homology domains and plays a role in the formation and isomerization of disulfide bonds (7,8). |
| 存放说明 | -20C |
| 参考文献 | 1 . Rajagopalan, S. et al. (1994) Science 263, 387-390. 2 . Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128. 3 . Rüder, C. et al. (2009) J Clin Invest 119, 2184-203. 4 . Williams, D.B. (2006) J. Cell Sci. 119, 615-623. 5 . Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8. 6 . Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32. 7 . Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6. 8 . Mazzarella, RA et al. (1990) J Biol Chem 265(2), 1094-101. 9 . Reimer, T.A. et al. (2005) BMC Cancer 5, 47. 10 . Satoh, M et al. (2005) Cell Stress Chaperones 10(4), 278-84 11 . Wolf, J. et al. (2010) FASEB J 24, 4000-19. 12 . Engelsberg, A. et al. (2003) J Biol Chem 278, 22998-3007. 13 . Bock, J.B. et al. (2001) Nature 409, 839-41. 14 . Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5. 15 . Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11. 16 . Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71. 17 . Mallard, F. et al. (2002) J Cell Biol 156, 653-64. 18 . Perera, H.K. et al. (2003) Mol Biol Cell 14, 2946-58. 19 . Shewan, A.M. et al. (2003) Mol Biol Cell 14, 973-86. 20 . Martín-Martín, B. et al. (2000) Blood 96, 2574-83. 21 . Wendler, F. et al. (2001) Mol Biol Cell 12, 1699-709. 22 . Kuliawat, R. et al. (2004) Mol Biol Cell 15, 1690-701. |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Western blot analysis of extracts from various cell lines using Syntaxin 6 (C34B2) Rabbit mAb. | |
Confocal immunofluorescent analysis of MCF-7 cells using Syntaxin 6 (C34B2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Calnexin (C5C9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb. | |
Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of PANC-1 cells using ERp72 (D70D12) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using ERp72 (D70D12) XP® Rabbit mAb. | |
Flow cytometric analysis of Hep G2 cells using ERp72 (D70D12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb. | |
Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (red). |
